Basic Concept of Biotechnology
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Basic Concept of Biotechnology
Biomolecules
Biomolecules Carbohydrates Classification of Carbohydrates Structure of Monosaccharide’s Structure of Di Saccharides and Polysaccharides Importance of carbohydrates Rare sugars Nucleic Acids Structure of Nucleic Acids Watson and Crick’s double helix structure of DNA Tautomerism Nucleosides and nucleotides Proteins - Biomolecules Amino Acids and Classification of Amino Acids Non-proteinogenic amino acids and its role Classification of Proteins Structure of Proteins Biological Importance of Proteins Lipids and Classification of Lipids Structure of lipids Fatty Acids Triacylglycerols Phospholipids Sterols and sterol estersComputer Applications and Biostatistics
Computer Applications and Biostatistics Information and computer technology Biostatistics Types of Data - Biostatistics Measures of Central Tendencies - Biostatistics Types of Distribution - Biostatistics Applications of Standard Error of Mean Confidence Interval (CI) Null Hypothesis Level of Significance Outliers One tailed and two tailed Test Importance of Sample Size Determination Factors Influencing Sample Size Include Sample Size Determination and Variance Estimate How to Choose an Appropriate Statistical Test Non-parametric tests used for analysis of different types of data Common problems faced by researcher in any trial and how to address them Biological data and its diversity Application of computer and statistics in biologyMacromolecules and Analytical Techniques
Macromolecules and Analytical Techniques Macromolecules Centrifugation - Analysis of biomolecules Chromatography: Types, Principle, Application - Analysis of biomolecules Electrophoresis - Analysis of biomolecules Spectroscopy - Analysis of biomolecules UV Visible spectroscopy - Analysis of biomolecules Infra Red Spectropscopy (IR spectroscopy) - Analysis of biomolecules Fluorometry - Analysis of biomolecules Mass Spectrometry - Analysis of biomolecules Nuclear Magnetic Resonance (NMR) spectroscopy - Analysis of biomoleculesPlant Molecular Farming A Promising Stratergy in Biotechnology
Plant Molecular Farming: A Promising Stratergy in Biotechnology Plants as Expression Systems Types of Biomolecules and recent examples Plant made pharmaceuticals (PMPs) Plant made nutritional compounds (PMNs) Plant made vaccines (PMVs) Plant made plastics (PMPs) Plant made industrial compounds (PMIs) The plant endomembrane system Subcellular Targeting of proteins in plants Different plant species as platform for molecular farming Plant Transformation Optimization of transgene expression in host plant Downstream processing of recombinant proteins Use of affinity tags for purification Use of non-chromatographic methods Biosafety and regulatory issuesPlant Transgenics Genetic Engineering Approch to Devlop Biotic Stress Resistance Plants
Plant Transgenics: Genetic Engineering Approch to Devlop Biotic Stress Resistance Plants The Power of Biotechnology Strategies for Competitiveness - Biotechnology Tools of Genetic Engineering in Plants DNA Delivery Systems - Plant Transgenics The Selection and Analysis of Transformants - Plant Transgenics Modification of regulatory elements of marker genes - Plant Transgenics More ‘Friendly’ Selectable Markers: the Positive Selection Method - Plant Transgenics Biotic Stress Virus Resistance RNAi Technology Fungal Resistance Bacterial Resistance Nematode ResistanceAnimal Biotechnology
Animal Biotechnology Cell Culture - Animal Biotechnology Criteria for Subculture - Animal Biotechnology Culture Conditions and Mammalian Cell - Animal Biotechnology Aseptic Techniques - Animal Biotechnology Cryopreservation Medium Protocol for Cryopreserving Cultured Cells Protocol for Thawing Frozen Cells Biological Contamination Protocol for Microbial Decontamination Protocol for Treating Mycoplasma contaminated Cell Cultures with BM Cyclin Cell Viability (quantitation and cytotoxicity) Factors to Consider When Choosing a Cell Viability Assay Cell Viability Assays that Measure ATP and Metabolic Capacity Cytotoxicity Assays: Determining the Number of Live and Dead Cells Trypan Blue Concentrating Cells Cell Proliferation - Animal Biotechnology Cell Cycle - Animal Biotechnology Cytotoxicity Applications of cell culture What are stem cells, and why are they important? What are the unique properties of all stem cells? Embryonic stem cells grown in the laboratory Similarities and Differences between Embryonic and Adult stem cells Potential uses of human stem cells and the obstacles Genetic manipulation of animals (animal models in research) Animal Breeding and Transgenic Animals Methods of genetic manipulation in animals A brief history of cloning Cloning Use of Animals in Research Improving Agricultural Products with Transgenics Genetically Engineered Insulin Gene Therapy Monoclonal Antibodies Economic impact in developing countries - Animal Biotechnology Ethical Issues(biosafety and regulatory issues) - Animal BiotechnologyMedical Biotechnology
Medical Biotechnology History of Medical Biotechnology Human Genome Project and its influence on medical biotechnology Detecting Genetic Diseases Biotech in the Hospital Role of biotechnology in diagnostics and Therapeutics Role of biotechnology in developing vaccines Treatments developed with the aid of biotechnology Other Applications of Biotechnology in Medical field Designer Babies - Medical Biotechnology New Biotech Breakthroughs about to change MedicineTools and Techniques in Biotechnology
Tools and Techniques in Biotechnology Basic Tools in the Biotechnology Measurement of Volume - Tools used in Biotechnology Measurement of Weight - Tools used in Biotechnology Measurement of pH - Tools used in Biotechnology Measurement of light - Tools used in Biotechnology Solution Preparation - Tools used in Biotechnology Microbiological techniques - Tools used in Biotechnology Preparation of biological samples for analysis - Tools used in Biotechnology Separation of macromolecules - Tools used in Biotechnology Manipulation of Nucleic Acids - Tools used in Biotechnology Techniques of Biotechnology and Innovations Isolation of Nucleic Acid (DNA) - Techniques of Biotechnology and Innovations Plasmid Isolation - Techniques of Biotechnology and Innovations RNA Isolation and mRNA Purification - Techniques of Biotechnology and Innovations Gene Isolation - Techniques of Biotechnology and Innovations Molecular Mapping - Techniques of Biotechnology and Innovations Genomic DNA Library Construction - Techniques of Biotechnology and Innovations Transformation Techniques - Techniques of Biotechnology and Innovations Direct Gene Transformation - Techniques of Biotechnology and Innovations Labeling Methods - Techniques of Biotechnology and Innovations Southern Blotting Technique - Techniques of Biotechnology and Innovations In Situ Hybridization (ISH) - Techniques of Biotechnology and Innovations Polymerase Chain Reaction(PCR) - Techniques of Biotechnology and Innovations Fluorescence In Situ Hybridization (FISH) - Techniques of Biotechnology and Innovations DNA Sequencing Techniques - Techniques of Biotechnology and Innovations Microarray - Techniques of Biotechnology and Innovations Next Generation Sequencing (NGS) - Techniques of Biotechnology and InnovationsAntibiotics Microbial Sources Production and Optimization
Antibiotics Antibacterial agents Actinomycetes Marine actinomycetes Streptomyces Identification of actinomycetes Metabolite production by marine Streptomyces Antagonistic marine actinomycetes in Indian peninsula Optimization of fermentation conditionsBiotechnology in Forensic Sciences
History of Forensic Genetic Typing Introduction - Biotechnology in Forensic Sciences Restriction Fragment Length Polymorphism (RFLP) Amplified Fragment Length Polymorphism (AMP-FLP) Polymerase Chain Reaction (PCR) - Biotechnology in Forensic Sciences Short Tandem Repeats (STRs) - Biotechnology in Forensic Sciences Y-Short Tandem Repeats X-chromosomal short tandem repeats (ChrX STRs) mtDNA in forensic science Microbial Forensics Non-human DNA Forensics
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