Basic Concept of Biotechnology

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Basic Concept of Biotechnology

Biomolecules


=> Biomolecules
=> Carbohydrates
=> Classification of Carbohydrates
=> Structure of Monosaccharide’s
=> Structure of Di Saccharides and Polysaccharides
=> Importance of carbohydrates
=> Rare sugars
=> Nucleic Acids
=> Structure of Nucleic Acids
=> Watson and Crick’s double helix structure of DNA
=> Tautomerism
=> Nucleosides and nucleotides
=> Proteins - Biomolecules
=> Amino Acids and Classification of Amino Acids
=> Non-proteinogenic amino acids and its role
=> Classification of Proteins
=> Structure of Proteins
=> Biological Importance of Proteins
=> Lipids and Classification of Lipids
=> Structure of lipids
=> Fatty Acids
=> Triacylglycerols
=> Phospholipids
=> Sterols and sterol esters

Computer Applications and Biostatistics


=> Computer Applications and Biostatistics
=> Information and computer technology
=> Biostatistics
=> Types of Data - Biostatistics
=> Measures of Central Tendencies - Biostatistics
=> Types of Distribution - Biostatistics
=> Applications of Standard Error of Mean
=> Confidence Interval (CI)
=> Null Hypothesis
=> Level of Significance
=> Outliers
=> One tailed and two tailed Test
=> Importance of Sample Size Determination
=> Factors Influencing Sample Size Include
=> Sample Size Determination and Variance Estimate
=> How to Choose an Appropriate Statistical Test
=> Non-parametric tests used for analysis of different types of data
=> Common problems faced by researcher in any trial and how to address them
=> Biological data and its diversity
=> Application of computer and statistics in biology

Macromolecules and Analytical Techniques


=> Macromolecules and Analytical Techniques
=> Macromolecules
=> Centrifugation - Analysis of biomolecules
=> Chromatography: Types, Principle, Application - Analysis of biomolecules
=> Electrophoresis - Analysis of biomolecules
=> Spectroscopy - Analysis of biomolecules
=> UV Visible spectroscopy - Analysis of biomolecules
=> Infra Red Spectropscopy (IR spectroscopy) - Analysis of biomolecules
=> Fluorometry - Analysis of biomolecules
=> Mass Spectrometry - Analysis of biomolecules
=> Nuclear Magnetic Resonance (NMR) spectroscopy - Analysis of biomolecules

Plant Molecular Farming A Promising Stratergy in Biotechnology


=> Plant Molecular Farming: A Promising Stratergy in Biotechnology
=> Plants as Expression Systems
=> Types of Biomolecules and recent examples
=> Plant made pharmaceuticals (PMPs)
=> Plant made nutritional compounds (PMNs)
=> Plant made vaccines (PMVs)
=> Plant made plastics (PMPs)
=> Plant made industrial compounds (PMIs)
=> The plant endomembrane system
=> Subcellular Targeting of proteins in plants
=> Different plant species as platform for molecular farming
=> Plant Transformation
=> Optimization of transgene expression in host plant
=> Downstream processing of recombinant proteins
=> Use of affinity tags for purification
=> Use of non-chromatographic methods
=> Biosafety and regulatory issues

Plant Transgenics Genetic Engineering Approch to Devlop Biotic Stress Resistance Plants


=> Plant Transgenics: Genetic Engineering Approch to Devlop Biotic Stress Resistance Plants
=> The Power of Biotechnology
=> Strategies for Competitiveness - Biotechnology
=> Tools of Genetic Engineering in Plants
=> DNA Delivery Systems - Plant Transgenics
=> The Selection and Analysis of Transformants - Plant Transgenics
=> Modification of regulatory elements of marker genes - Plant Transgenics
=> More ‘Friendly’ Selectable Markers: the Positive Selection Method - Plant Transgenics
=> Biotic Stress
=> Virus Resistance
=> RNAi Technology
=> Fungal Resistance
=> Bacterial Resistance
=> Nematode Resistance

Animal Biotechnology


=> Animal Biotechnology
=> Cell Culture - Animal Biotechnology
=> Criteria for Subculture - Animal Biotechnology
=> Culture Conditions and Mammalian Cell - Animal Biotechnology
=> Aseptic Techniques - Animal Biotechnology
=> Cryopreservation Medium
=> Protocol for Cryopreserving Cultured Cells
=> Protocol for Thawing Frozen Cells
=> Biological Contamination
=> Protocol for Microbial Decontamination
=> Protocol for Treating Mycoplasma contaminated Cell Cultures with BM Cyclin
=> Cell Viability (quantitation and cytotoxicity)
=> Factors to Consider When Choosing a Cell Viability Assay
=> Cell Viability Assays that Measure ATP and Metabolic Capacity
=> Cytotoxicity Assays: Determining the Number of Live and Dead Cells
=> Trypan Blue
=> Concentrating Cells
=> Cell Proliferation - Animal Biotechnology
=> Cell Cycle - Animal Biotechnology
=> Cytotoxicity
=> Applications of cell culture
=> What are stem cells, and why are they important?
=> What are the unique properties of all stem cells?
=> Embryonic stem cells grown in the laboratory
=> Similarities and Differences between Embryonic and Adult stem cells
=> Potential uses of human stem cells and the obstacles
=> Genetic manipulation of animals (animal models in research)
=> Animal Breeding and Transgenic Animals
=> Methods of genetic manipulation in animals
=> A brief history of cloning
=> Cloning
=> Use of Animals in Research
=> Improving Agricultural Products with Transgenics
=> Genetically Engineered Insulin
=> Gene Therapy
=> Monoclonal Antibodies
=> Economic impact in developing countries - Animal Biotechnology
=> Ethical Issues(biosafety and regulatory issues) - Animal Biotechnology

Medical Biotechnology


=> Medical Biotechnology
=> History of Medical Biotechnology
=> Human Genome Project and its influence on medical biotechnology
=> Detecting Genetic Diseases
=> Biotech in the Hospital
=> Role of biotechnology in diagnostics and Therapeutics
=> Role of biotechnology in developing vaccines
=> Treatments developed with the aid of biotechnology
=> Other Applications of Biotechnology in Medical field
=> Designer Babies - Medical Biotechnology
=> New Biotech Breakthroughs about to change Medicine

Tools and Techniques in Biotechnology


=> Tools and Techniques in Biotechnology
=> Basic Tools in the Biotechnology
=> Measurement of Volume - Tools used in Biotechnology
=> Measurement of Weight - Tools used in Biotechnology
=> Measurement of pH - Tools used in Biotechnology
=> Measurement of light - Tools used in Biotechnology
=> Solution Preparation - Tools used in Biotechnology
=> Microbiological techniques - Tools used in Biotechnology
=> Preparation of biological samples for analysis - Tools used in Biotechnology
=> Separation of macromolecules - Tools used in Biotechnology
=> Manipulation of Nucleic Acids - Tools used in Biotechnology
=> Techniques of Biotechnology and Innovations
=> Isolation of Nucleic Acid (DNA) - Techniques of Biotechnology and Innovations
=> Plasmid Isolation - Techniques of Biotechnology and Innovations
=> RNA Isolation and mRNA Purification - Techniques of Biotechnology and Innovations
=> Gene Isolation - Techniques of Biotechnology and Innovations
=> Molecular Mapping - Techniques of Biotechnology and Innovations
=> Genomic DNA Library Construction - Techniques of Biotechnology and Innovations
=> Transformation Techniques - Techniques of Biotechnology and Innovations
=> Direct Gene Transformation - Techniques of Biotechnology and Innovations
=> Labeling Methods - Techniques of Biotechnology and Innovations
=> Southern Blotting Technique - Techniques of Biotechnology and Innovations
=> In Situ Hybridization (ISH) - Techniques of Biotechnology and Innovations
=> Polymerase Chain Reaction(PCR) - Techniques of Biotechnology and Innovations
=> Fluorescence In Situ Hybridization (FISH) - Techniques of Biotechnology and Innovations
=> DNA Sequencing Techniques - Techniques of Biotechnology and Innovations
=> Microarray - Techniques of Biotechnology and Innovations
=> Next Generation Sequencing (NGS) - Techniques of Biotechnology and Innovations

Antibiotics Microbial Sources Production and Optimization


=> Antibiotics
=> Antibacterial agents
=> Actinomycetes
=> Marine actinomycetes
=> Streptomyces
=> Identification of actinomycetes
=> Metabolite production by marine Streptomyces
=> Antagonistic marine actinomycetes in Indian peninsula
=> Optimization of fermentation conditions

Biotechnology in Forensic Sciences


=> History of Forensic Genetic Typing
=> Introduction - Biotechnology in Forensic Sciences
=> Restriction Fragment Length Polymorphism (RFLP)
=> Amplified Fragment Length Polymorphism (AMP-FLP)
=> Polymerase Chain Reaction (PCR) - Biotechnology in Forensic Sciences
=> Short Tandem Repeats (STRs) - Biotechnology in Forensic Sciences
=> Y-Short Tandem Repeats
=> X-chromosomal short tandem repeats (ChrX STRs)
=> mtDNA in forensic science
=> Microbial Forensics
=> Non-human DNA Forensics



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