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Chapter: Basic Concept of Biotechnology - Macromolecules and Analytical Techniques

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UV Visible spectroscopy - Analysis of biomolecules

The electronic spectra of organic molecules which are found in UV region (100nm-400nm) and visible region (400nm-750nm) can be studied by the UV-Visible spectrophotometer.

UV-Visible spectroscopy

The electronic spectra of organic molecules which are found in UV region (100nm-400nm) and visible region (400nm-750nm) can be studied by the UV-Visible spectrophotometer. UV and Visible radiations absorbed by the molecules will bring transition of outer shell electrons. When the sample is irradiated with the UV - Visible radiation if a particular electronic transition matches the energy of a certain band of UV - Visible, it will be absorbed. The remaining UV - Visible light passes through the sample and is observed with the gaps in the spectrum region and is called absorption spectrum.

 

Applications

 

·        To determine the absorbance or transmission of characteristic wavelengths of radiant energy (light) by a chemical species in solution.

 

·        Identify organic compounds by determining the absorption maximum.

 

·        Used for color determination within the spectral range

 

·        Concentration of impurities

 

·        To study the rate of a reaction

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