Microbiological
techniques
Specialized
equipment is required to isolate, transfer, and grow up cultures of microbes
and tissues in the laboratory.
A. Autoclaves –Autoclaves
are machines that achieve a high internaltemperature and pressure and are used
to sterilize solutions and glassware. The kitchen pressure cooker achieves the
same results and can be used instead of an autoclave.
B. Cell Culture Hood –A
biological safety or cell culture hood filterssmall particles out of the air in
order to avoid contamination of cultures or sterile media. The filters are
similar to those used to decontaminate air for operating rooms in hospitals or
clean rooms used in the semiconductor industry.
C. Fermentors –Fermentors
are used to grow up a large quantity of cellswith automatically controlled pH
and levels of oxygen and other nutrients.
D. Since
most cells are generally too small to be seen with the naked eye, microscopes
are used to magnify their images. Light or Bright field microscopes and
inverted microscopes are the most common types found in biotechnology
laboratories.
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