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Chapter: Basic Concept of Biotechnology - Tools and Techniques in Biotechnology

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Measurement of pH - Tools used in Biotechnology

Most solutions prepared in the biological laboratory must have a carefully controlled pH.

Measurement of pH

Most solutions prepared in the biological laboratory must have a carefully controlled pH. Buffers are prepared by adjustment to a specific pH with strong acid and base solutions, using a meter to monitor the pH. A pH meter is a volt meter that measures the electrical potential between two electrodes. One electrode is in contact with your solution, and the other is in contact with a reference solution. Usually both of these electrodes are combined in a single pH probe that you place in your solution. These meters can read to the nearest 0.1 pH unit, but require frequent calibration with reference buffers of known pH.

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