Concentrating
Cells:
To
concentrate cells from a suspension culture (or resuspended cells from
monolayer culture):
1.
Transfer the cell suspension to a
sterile centrifuge tube of appropriate size andcentrifuge for 10 minutes at 800
~ g.
Note: Certain
cell lines are very sensitive to centrifugal force.
2.
Carefully remove the supernatant without
disturbing the cell pellet.
3.
Add the desired volume of fresh medium
gently to the side of the tube and slowly pipette up and down 2 to 3 times to
resuspend the cell pellet.
4.
Transfer the cells to the desired,
sterile container.
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