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Basic Concept of Biotechnology -

Basic Concept of Biotechnology

Biomolecules


-:- Biomolecules
-:- Carbohydrates
-:- Classification of Carbohydrates
-:- Structure of Monosaccharide’s
-:- Structure of Di Saccharides and Polysaccharides
-:- Importance of carbohydrates
-:- Rare sugars
-:- Nucleic Acids
-:- Structure of Nucleic Acids
-:- Watson and Crick’s double helix structure of DNA
-:- Tautomerism
-:- Nucleosides and nucleotides
-:- Proteins - Biomolecules
-:- Amino Acids and Classification of Amino Acids
-:- Non-proteinogenic amino acids and its role
-:- Classification of Proteins
-:- Structure of Proteins
-:- Biological Importance of Proteins
-:- Lipids and Classification of Lipids
-:- Structure of lipids
-:- Fatty Acids
-:- Triacylglycerols
-:- Phospholipids
-:- Sterols and sterol esters

Computer Applications and Biostatistics


-:- Computer Applications and Biostatistics
-:- Information and computer technology
-:- Biostatistics
-:- Types of Data - Biostatistics
-:- Measures of Central Tendencies - Biostatistics
-:- Types of Distribution - Biostatistics
-:- Applications of Standard Error of Mean
-:- Confidence Interval (CI)
-:- Null Hypothesis
-:- Level of Significance
-:- Outliers
-:- One tailed and two tailed Test
-:- Importance of Sample Size Determination
-:- Factors Influencing Sample Size Include
-:- Sample Size Determination and Variance Estimate
-:- How to Choose an Appropriate Statistical Test
-:- Non-parametric tests used for analysis of different types of data
-:- Common problems faced by researcher in any trial and how to address them
-:- Biological data and its diversity
-:- Application of computer and statistics in biology

Macromolecules and Analytical Techniques


-:- Macromolecules and Analytical Techniques
-:- Macromolecules
-:- Centrifugation - Analysis of biomolecules
-:- Chromatography: Types, Principle, Application - Analysis of biomolecules
-:- Electrophoresis - Analysis of biomolecules
-:- Spectroscopy - Analysis of biomolecules
-:- UV Visible spectroscopy - Analysis of biomolecules
-:- Infra Red Spectropscopy (IR spectroscopy) - Analysis of biomolecules
-:- Fluorometry - Analysis of biomolecules
-:- Mass Spectrometry - Analysis of biomolecules
-:- Nuclear Magnetic Resonance (NMR) spectroscopy - Analysis of biomolecules

Plant Molecular Farming A Promising Stratergy in Biotechnology


-:- Plant Molecular Farming: A Promising Stratergy in Biotechnology
-:- Plants as Expression Systems
-:- Types of Biomolecules and recent examples
-:- Plant made pharmaceuticals (PMPs)
-:- Plant made nutritional compounds (PMNs)
-:- Plant made vaccines (PMVs)
-:- Plant made plastics (PMPs)
-:- Plant made industrial compounds (PMIs)
-:- The plant endomembrane system
-:- Subcellular Targeting of proteins in plants
-:- Different plant species as platform for molecular farming
-:- Plant Transformation
-:- Optimization of transgene expression in host plant
-:- Downstream processing of recombinant proteins
-:- Use of affinity tags for purification
-:- Use of non-chromatographic methods
-:- Biosafety and regulatory issues

Plant Transgenics Genetic Engineering Approch to Devlop Biotic Stress Resistance Plants


-:- Plant Transgenics: Genetic Engineering Approch to Devlop Biotic Stress Resistance Plants
-:- The Power of Biotechnology
-:- Strategies for Competitiveness - Biotechnology
-:- Tools of Genetic Engineering in Plants
-:- DNA Delivery Systems - Plant Transgenics
-:- The Selection and Analysis of Transformants - Plant Transgenics
-:- Modification of regulatory elements of marker genes - Plant Transgenics
-:- More ‘Friendly’ Selectable Markers: the Positive Selection Method - Plant Transgenics
-:- Biotic Stress
-:- Virus Resistance
-:- RNAi Technology
-:- Fungal Resistance
-:- Bacterial Resistance
-:- Nematode Resistance

Animal Biotechnology


-:- Animal Biotechnology
-:- Cell Culture - Animal Biotechnology
-:- Criteria for Subculture - Animal Biotechnology
-:- Culture Conditions and Mammalian Cell - Animal Biotechnology
-:- Aseptic Techniques - Animal Biotechnology
-:- Cryopreservation Medium
-:- Protocol for Cryopreserving Cultured Cells
-:- Protocol for Thawing Frozen Cells
-:- Biological Contamination
-:- Protocol for Microbial Decontamination
-:- Protocol for Treating Mycoplasma contaminated Cell Cultures with BM Cyclin
-:- Cell Viability (quantitation and cytotoxicity)
-:- Factors to Consider When Choosing a Cell Viability Assay
-:- Cell Viability Assays that Measure ATP and Metabolic Capacity
-:- Cytotoxicity Assays: Determining the Number of Live and Dead Cells
-:- Trypan Blue
-:- Concentrating Cells
-:- Cell Proliferation - Animal Biotechnology
-:- Cell Cycle - Animal Biotechnology
-:- Cytotoxicity
-:- Applications of cell culture
-:- What are stem cells, and why are they important?
-:- What are the unique properties of all stem cells?
-:- Embryonic stem cells grown in the laboratory
-:- Similarities and Differences between Embryonic and Adult stem cells
-:- Potential uses of human stem cells and the obstacles
-:- Genetic manipulation of animals (animal models in research)
-:- Animal Breeding and Transgenic Animals
-:- Methods of genetic manipulation in animals
-:- A brief history of cloning
-:- Cloning
-:- Use of Animals in Research
-:- Improving Agricultural Products with Transgenics
-:- Genetically Engineered Insulin
-:- Gene Therapy
-:- Monoclonal Antibodies
-:- Economic impact in developing countries - Animal Biotechnology
-:- Ethical Issues(biosafety and regulatory issues) - Animal Biotechnology

Medical Biotechnology


-:- Medical Biotechnology
-:- History of Medical Biotechnology
-:- Human Genome Project and its influence on medical biotechnology
-:- Detecting Genetic Diseases
-:- Biotech in the Hospital
-:- Role of biotechnology in diagnostics and Therapeutics
-:- Role of biotechnology in developing vaccines
-:- Treatments developed with the aid of biotechnology
-:- Other Applications of Biotechnology in Medical field
-:- Designer Babies - Medical Biotechnology
-:- New Biotech Breakthroughs about to change Medicine

Tools and Techniques in Biotechnology


-:- Tools and Techniques in Biotechnology
-:- Basic Tools in the Biotechnology
-:- Measurement of Volume - Tools used in Biotechnology
-:- Measurement of Weight - Tools used in Biotechnology
-:- Measurement of pH - Tools used in Biotechnology
-:- Measurement of light - Tools used in Biotechnology
-:- Solution Preparation - Tools used in Biotechnology
-:- Microbiological techniques - Tools used in Biotechnology
-:- Preparation of biological samples for analysis - Tools used in Biotechnology
-:- Separation of macromolecules - Tools used in Biotechnology
-:- Manipulation of Nucleic Acids - Tools used in Biotechnology
-:- Techniques of Biotechnology and Innovations
-:- Isolation of Nucleic Acid (DNA) - Techniques of Biotechnology and Innovations
-:- Plasmid Isolation - Techniques of Biotechnology and Innovations
-:- RNA Isolation and mRNA Purification - Techniques of Biotechnology and Innovations
-:- Gene Isolation - Techniques of Biotechnology and Innovations
-:- Molecular Mapping - Techniques of Biotechnology and Innovations
-:- Genomic DNA Library Construction - Techniques of Biotechnology and Innovations
-:- Transformation Techniques - Techniques of Biotechnology and Innovations
-:- Direct Gene Transformation - Techniques of Biotechnology and Innovations
-:- Labeling Methods - Techniques of Biotechnology and Innovations
-:- Southern Blotting Technique - Techniques of Biotechnology and Innovations
-:- In Situ Hybridization (ISH) - Techniques of Biotechnology and Innovations
-:- Polymerase Chain Reaction(PCR) - Techniques of Biotechnology and Innovations
-:- Fluorescence In Situ Hybridization (FISH) - Techniques of Biotechnology and Innovations
-:- DNA Sequencing Techniques - Techniques of Biotechnology and Innovations
-:- Microarray - Techniques of Biotechnology and Innovations
-:- Next Generation Sequencing (NGS) - Techniques of Biotechnology and Innovations

Antibiotics Microbial Sources Production and Optimization


-:- Antibiotics
-:- Antibacterial agents
-:- Actinomycetes
-:- Marine actinomycetes
-:- Streptomyces
-:- Identification of actinomycetes
-:- Metabolite production by marine Streptomyces
-:- Antagonistic marine actinomycetes in Indian peninsula
-:- Optimization of fermentation conditions

Biotechnology in Forensic Sciences


-:- History of Forensic Genetic Typing
-:- Introduction - Biotechnology in Forensic Sciences
-:- Restriction Fragment Length Polymorphism (RFLP)
-:- Amplified Fragment Length Polymorphism (AMP-FLP)
-:- Polymerase Chain Reaction (PCR) - Biotechnology in Forensic Sciences
-:- Short Tandem Repeats (STRs) - Biotechnology in Forensic Sciences
-:- Y-Short Tandem Repeats
-:- X-chromosomal short tandem repeats (ChrX STRs)
-:- mtDNA in forensic science
-:- Microbial Forensics
-:- Non-human DNA Forensics