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Basic Concept of Biotechnology
Online Study Material, Lecturing Notes, Assignment, Reference, Wiki and important questions and answers
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Biotechnology
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Biomolecules
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Biomolecules
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Carbohydrates
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Classification of Carbohydrates
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Structure of Monosaccharide’s
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Structure of Di Saccharides and Polysaccharides
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Importance of carbohydrates
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Rare sugars
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Nucleic Acids
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Structure of Nucleic Acids
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Watson and Crick’s double helix structure of DNA
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Tautomerism
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Nucleosides and nucleotides
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Proteins - Biomolecules
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Amino Acids and Classification of Amino Acids
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Non-proteinogenic amino acids and its role
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Classification of Proteins
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Structure of Proteins
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Biological Importance of Proteins
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Lipids and Classification of Lipids
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Structure of lipids
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Fatty Acids
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Triacylglycerols
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Phospholipids
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Sterols and sterol esters
Computer Applications and Biostatistics
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Computer Applications and Biostatistics
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Information and computer technology
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Biostatistics
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Types of Data - Biostatistics
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Measures of Central Tendencies - Biostatistics
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Types of Distribution - Biostatistics
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Applications of Standard Error of Mean
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Confidence Interval (CI)
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Null Hypothesis
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Level of Significance
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Outliers
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One tailed and two tailed Test
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Importance of Sample Size Determination
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Factors Influencing Sample Size Include
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Sample Size Determination and Variance Estimate
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How to Choose an Appropriate Statistical Test
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Non-parametric tests used for analysis of different types of data
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Common problems faced by researcher in any trial and how to address them
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Biological data and its diversity
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Application of computer and statistics in biology
Macromolecules and Analytical Techniques
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Macromolecules and Analytical Techniques
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Macromolecules
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Centrifugation - Analysis of biomolecules
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Chromatography: Types, Principle, Application - Analysis of biomolecules
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Electrophoresis - Analysis of biomolecules
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Spectroscopy - Analysis of biomolecules
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UV Visible spectroscopy - Analysis of biomolecules
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Infra Red Spectropscopy (IR spectroscopy) - Analysis of biomolecules
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Fluorometry - Analysis of biomolecules
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Mass Spectrometry - Analysis of biomolecules
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Nuclear Magnetic Resonance (NMR) spectroscopy - Analysis of biomolecules
Plant Molecular Farming A Promising Stratergy in Biotechnology
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Plant Molecular Farming: A Promising Stratergy in Biotechnology
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Plants as Expression Systems
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Types of Biomolecules and recent examples
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Plant made pharmaceuticals (PMPs)
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Plant made nutritional compounds (PMNs)
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Plant made vaccines (PMVs)
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Plant made plastics (PMPs)
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Plant made industrial compounds (PMIs)
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The plant endomembrane system
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Subcellular Targeting of proteins in plants
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Different plant species as platform for molecular farming
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Plant Transformation
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Optimization of transgene expression in host plant
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Downstream processing of recombinant proteins
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Use of affinity tags for purification
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Use of non-chromatographic methods
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Biosafety and regulatory issues
Plant Transgenics Genetic Engineering Approch to Devlop Biotic Stress Resistance Plants
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Plant Transgenics: Genetic Engineering Approch to Devlop Biotic Stress Resistance Plants
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The Power of Biotechnology
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Strategies for Competitiveness - Biotechnology
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Tools of Genetic Engineering in Plants
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DNA Delivery Systems - Plant Transgenics
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The Selection and Analysis of Transformants - Plant Transgenics
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Modification of regulatory elements of marker genes - Plant Transgenics
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More ‘Friendly’ Selectable Markers: the Positive Selection Method - Plant Transgenics
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Biotic Stress
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Virus Resistance
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RNAi Technology
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Fungal Resistance
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Bacterial Resistance
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Nematode Resistance
Animal Biotechnology
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Animal Biotechnology
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Cell Culture - Animal Biotechnology
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Criteria for Subculture - Animal Biotechnology
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Culture Conditions and Mammalian Cell - Animal Biotechnology
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Aseptic Techniques - Animal Biotechnology
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Cryopreservation Medium
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Protocol for Cryopreserving Cultured Cells
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Protocol for Thawing Frozen Cells
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Biological Contamination
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Protocol for Microbial Decontamination
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Protocol for Treating Mycoplasma contaminated Cell Cultures with BM Cyclin
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Cell Viability (quantitation and cytotoxicity)
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Factors to Consider When Choosing a Cell Viability Assay
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Cell Viability Assays that Measure ATP and Metabolic Capacity
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Cytotoxicity Assays: Determining the Number of Live and Dead Cells
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Trypan Blue
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Concentrating Cells
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Cell Proliferation - Animal Biotechnology
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Cell Cycle - Animal Biotechnology
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Cytotoxicity
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Applications of cell culture
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What are stem cells, and why are they important?
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What are the unique properties of all stem cells?
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Embryonic stem cells grown in the laboratory
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Similarities and Differences between Embryonic and Adult stem cells
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Potential uses of human stem cells and the obstacles
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Genetic manipulation of animals (animal models in research)
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Animal Breeding and Transgenic Animals
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Methods of genetic manipulation in animals
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A brief history of cloning
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Cloning
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Use of Animals in Research
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Improving Agricultural Products with Transgenics
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Genetically Engineered Insulin
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Gene Therapy
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Monoclonal Antibodies
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Economic impact in developing countries - Animal Biotechnology
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Ethical Issues(biosafety and regulatory issues) - Animal Biotechnology
Medical Biotechnology
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Medical Biotechnology
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History of Medical Biotechnology
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Human Genome Project and its influence on medical biotechnology
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Detecting Genetic Diseases
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Biotech in the Hospital
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Role of biotechnology in diagnostics and Therapeutics
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Role of biotechnology in developing vaccines
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Treatments developed with the aid of biotechnology
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Other Applications of Biotechnology in Medical field
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Designer Babies - Medical Biotechnology
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New Biotech Breakthroughs about to change Medicine
Tools and Techniques in Biotechnology
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Tools and Techniques in Biotechnology
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Basic Tools in the Biotechnology
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Measurement of Volume - Tools used in Biotechnology
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Measurement of Weight - Tools used in Biotechnology
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Measurement of pH - Tools used in Biotechnology
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Measurement of light - Tools used in Biotechnology
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Solution Preparation - Tools used in Biotechnology
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Microbiological techniques - Tools used in Biotechnology
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Preparation of biological samples for analysis - Tools used in Biotechnology
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Separation of macromolecules - Tools used in Biotechnology
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Manipulation of Nucleic Acids - Tools used in Biotechnology
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Techniques of Biotechnology and Innovations
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Isolation of Nucleic Acid (DNA) - Techniques of Biotechnology and Innovations
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Plasmid Isolation - Techniques of Biotechnology and Innovations
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RNA Isolation and mRNA Purification - Techniques of Biotechnology and Innovations
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Gene Isolation - Techniques of Biotechnology and Innovations
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Molecular Mapping - Techniques of Biotechnology and Innovations
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Genomic DNA Library Construction - Techniques of Biotechnology and Innovations
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Transformation Techniques - Techniques of Biotechnology and Innovations
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Direct Gene Transformation - Techniques of Biotechnology and Innovations
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Labeling Methods - Techniques of Biotechnology and Innovations
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Southern Blotting Technique - Techniques of Biotechnology and Innovations
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In Situ Hybridization (ISH) - Techniques of Biotechnology and Innovations
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Polymerase Chain Reaction(PCR) - Techniques of Biotechnology and Innovations
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Fluorescence In Situ Hybridization (FISH) - Techniques of Biotechnology and Innovations
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DNA Sequencing Techniques - Techniques of Biotechnology and Innovations
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Microarray - Techniques of Biotechnology and Innovations
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Next Generation Sequencing (NGS) - Techniques of Biotechnology and Innovations
Antibiotics Microbial Sources Production and Optimization
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Antibiotics
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Antibacterial agents
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Actinomycetes
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Marine actinomycetes
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Streptomyces
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Identification of actinomycetes
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Metabolite production by marine Streptomyces
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Antagonistic marine actinomycetes in Indian peninsula
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Optimization of fermentation conditions
Biotechnology in Forensic Sciences
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History of Forensic Genetic Typing
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Introduction - Biotechnology in Forensic Sciences
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Restriction Fragment Length Polymorphism (RFLP)
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Amplified Fragment Length Polymorphism (AMP-FLP)
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Polymerase Chain Reaction (PCR) - Biotechnology in Forensic Sciences
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Short Tandem Repeats (STRs) - Biotechnology in Forensic Sciences
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Y-Short Tandem Repeats
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X-chromosomal short tandem repeats (ChrX STRs)
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mtDNA in forensic science
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Microbial Forensics
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Non-human DNA Forensics
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