Biotechnology

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Biotechnology

Recombinant DNA Technology


=> Tools of rDNA technology
=> Restriction Fragment Length Polymorphism (RFLP)
=> Vectors: Vehicles for cloning
=> Plasmids
=> Vectors based on bacteriophages
=> Making rDNA
=> Introduction of rDNA into host cells - Recombinant DNA Technology
=> Identification of Recombinants - Recombinant DNA Technology
=> Polymerase Chain Reaction (PCR)
=> Hybridisation Techniques - Recombinant DNA Technology
=> DNA Library
=> DNA Sequencing
=> Site-directed Mutagenesis

Protein Structure And Engineering


=> Introduction to the World of Proteins
=> 3-D Shape of Proteins
=> Structure-Function relationship in Proteins
=> Chymotrypsin, a proteolytic enzyme
=> Molecular Disease- Sickle cell anaemia
=> Protein Finger printing- Peptide Mapping
=> 2-D Gel Electrophoresis
=> Purification of Proteins
=> Characterisation of Proteins
=> Protein Based Products
=> Designing Proteins (Protein Engineering)

Genomics and Bioinformatics


=> Genomics and Bioinformatics
=> Genome Sequencing Projects
=> Gene prediction and counting
=> Genome Similarity, SNPs and Comparative Genomics
=> Functional Genomics
=> Fluorescence in situ hybridization
=> Microarray Technology
=> Proteomics and Types of Proteomics
=> History of Bioinformatics
=> Sequences and nomenclature - Bioinformatics
=> Different types of sequences - Bioinformatics
=> Information Sources - Bioinformatics
=> Analysis using Bioinformatics tools

Microbial Cell Culture and its Applications


=> Microbial Cell Culture and its Applications
=> Microbial culture techniques
=> Measurement and kinetics of microbial growth
=> Scale-up of microbial processes
=> Isolation of microbial products
=> Strain isolation, improvement and preservation
=> Applications of microbial culture technology
=> Biosafety issues in Microbial Technology

Plant Cell Culture and Applications


=> Plant Cell Culture and Applications
=> Cell and Tissue Culture Techniques
=> Applications of Cell and Tissue Culture
=> Gene transfer methods in plants
=> Transgenic plants with beneficial traits
=> Biotic and Abiotic stress tolerance
=> Biosafety in Plant Genetic Engineering

Animal Cell Culture and Applications


=> Animal Cell Culture and Applications
=> Animal Cell Culture Techniques
=> Characterization of Cell Lines
=> Methods of Gene Delivery into Cells
=> Scale-up of Animal Culture Process
=> Applications of Animal Cell culture
=> Stem Cell Technology
=> Tissue engineering



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