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Recombinant DNA Technology

Tools of rDNA technology
Restriction Fragment Length Polymorphism (RFLP)
Vectors: Vehicles for cloning
Vectors based on bacteriophages
Making rDNA
Introduction of rDNA into host cells - Recombinant DNA Technology
Identification of Recombinants - Recombinant DNA Technology
Polymerase Chain Reaction (PCR)
Hybridisation Techniques - Recombinant DNA Technology
DNA Library
DNA Sequencing
Site-directed Mutagenesis

Protein Structure And Engineering

Introduction to the World of Proteins
3-D Shape of Proteins
Structure-Function relationship in Proteins
Chymotrypsin, a proteolytic enzyme
Molecular Disease- Sickle cell anaemia
Protein Finger printing- Peptide Mapping
2-D Gel Electrophoresis
Purification of Proteins
Characterisation of Proteins
Protein Based Products
Designing Proteins (Protein Engineering)

Genomics and Bioinformatics

Genomics and Bioinformatics
Genome Sequencing Projects
Gene prediction and counting
Genome Similarity, SNPs and Comparative Genomics
Functional Genomics
Fluorescence in situ hybridization
Microarray Technology
Proteomics and Types of Proteomics
History of Bioinformatics
Sequences and nomenclature - Bioinformatics
Different types of sequences - Bioinformatics
Information Sources - Bioinformatics
Analysis using Bioinformatics tools

Microbial Cell Culture and its Applications

Microbial Cell Culture and its Applications
Microbial culture techniques
Measurement and kinetics of microbial growth
Scale-up of microbial processes
Isolation of microbial products
Strain isolation, improvement and preservation
Applications of microbial culture technology
Biosafety issues in Microbial Technology

Plant Cell Culture and Applications

Plant Cell Culture and Applications
Cell and Tissue Culture Techniques
Applications of Cell and Tissue Culture
Gene transfer methods in plants
Transgenic plants with beneficial traits
Biotic and Abiotic stress tolerance
Biosafety in Plant Genetic Engineering

Animal Cell Culture and Applications

Animal Cell Culture and Applications
Animal Cell Culture Techniques
Characterization of Cell Lines
Methods of Gene Delivery into Cells
Scale-up of Animal Culture Process
Applications of Animal Cell culture
Stem Cell Technology
Tissue engineering

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