Have you noticed the preparation of curd in our home? A little drop of curd turns the milk into curd after some time. What is responsible for this change? Why it Sours? The change is brought by Lactobacillus lactis, a bacterium present in the curd. The sourness is due to the formation of Lactic acid. Have you been a victim of Typhoid? It is a bacterial disease caused by Salmonella typhi, a bacterium. So we can consider this prokaryotic organism as friend and foe, due to their beneficial and harmful activities.
Robert Koch (1843–1910)
Robert Heinrich Hermann Koch was a German physician and microbiologist. He is considered as the founder of modern bacteriology. He identified the causal organism for Anthrax, Cholera and Tuberculosis. The experimental evidence for the concept of infection was proved by him (Koch’s postulates). He was awarded Nobel prize in Medicine/Physiology in the year 1905.
1829 C.G. Ehrenberg coined the term Bacterium
1884 Christian Gram introduced Gram staining method
1923 David H. Bergy published First edition of Bergey’s Manual
1928 Fredrick Griffith discovered Bacterial transformation
1952 Joshua Lederberg discovered of Plasmid
Bacteria are prokaryotic, unicellular, ubiq-uitous, microscopic organisms. The study of Bacteria is called Bacteriology. Bacteria were first discovered by a Dutch scientist, Anton van Leeuwenhoek in 1676 and were called “animalcules”.
• They are Prokaryotic organisms and lack nuclear membrane and membrane bound organelles.
• The Genetic material is called nucleoid or genophore or incipient nucleus
• The cell wall is made up of Polysaccharides and proteins
• Most of them lack chlorophyll, hence they are heterotrophic (Vibrio cholerae) but some are autotrophic and possess Bacteriochlorophyll (Chromatium)
• They reproduce vegetatively by Binary fission and endospore formation.
• They exhibit variations which are due to genetic recombination and is achieved through conjugation, transformation and transduction.
The shape and flagellation of the bacteria varies and is given in Figure 1.8
The bacterial cell reveals three layers (i) Capsule/Glycocalyx (ii) Cell wall and (iii) Cytoplasm (Figure 1.9)
Some bacteria are surrounded by a gelatinous substance which is composed of polysaccharides or polypeptide or both. A thick layer of glycocalyx bound tightly to the cell wall is called capsule. It protects cell from desiccation and antibiotics. The sticky nature helps them to attach to substrates like plant root surfaces, Human teeth and tissues. It helps to retain the nutrients in bacterial cell.
The bacterial cell wall is granular and is rigid. It provide protection and gives shape to the cell. The chemical composition of cell wall is rather complex and is made up of Peptidoglycan or mucopeptide ( N-acetyl glucosamine, N-acetyl muramic acid and peptide chain of 4 or 5 aminoacids). One of the most abundant polypeptide called porin is present and it helps in the diffusion of solutes.
The plasma membrane is made up of lipoprotein. It controls the entry and exit of small molecules and ions. The enzymes involved in the oxidation of metabolites (i.e., the respiratory chain) as well as the photosystems used in photosynthesis are present in the plasma membrane.
Cytoplasm is thick and semitransparent. It contains ribosomes and other cell inclusions. Cytoplasmic inclusions like glycogen, poly-β-hydroxybutyrate granules, sulphur granules and gas vesicles are present.
The bacterial chromosome is a single circular DNA molecule, tightly coiled and is not enclosed in a membrane as in Eukaryotes. This genetic material is called Nucleoid or Genophore. It is amazing to note that the DNA of E.coli which measures about 1mm long when uncoiled, contains all the genetic information of the organism. The DNA is not bound to histone proteins. The single chromosome or the DNA molecule is circular and at one point it is attached to the plasma membrane and it is believed that this attachment may help in the separation of two chromosomes after DNA replication.
Plasmids are extra chromosomal double stranded, circular, self-replicating, autonomous elements. They contain genes for fertility, antibiotic resistant and heavy metals. It also help in the production of bacteriocins and toxins which are not found in bacterial chromosome. The size of a plasmid varies from 1 to 500 kb usually plasmids contribute to about 0.5 to 5.0% of the total DNA of bacteria. The number of plasmids per cell varies. Plasmids are classified into different types based on the function. Some of them are F (Fertility) factor, R (Resistance) plasmids, Col (Colicin) plasmids, Ri (Root inducing) plasmids and Ti (Tumour inducing) plasmids.
These are localized infoldings of plasma membrane produced into the cell in the form of vesicles, tubules and lamellae. They are clumped and folded together to maximize their surface area and helps in respiration and in binary fission.
The ribosomes are the site of protein synthesis. The number of ribosome per cell varies from 10,000 to 15,000. The ribosomes are 70S type and consists of two subunits (50S and 30S). The ribosomes are held together by mRNA and form polyribosomes or polysomes.
Certain motile bacteria have numerous thin hair like processes of variable length emerge from the cell wall called flagella. It is 20–30 μm in diameter and 15 μm in length. The flagella of Eukaryotic cells contain 9+2 microtubles but each flagellum in bacteria is made up of a single fibril. Flagella are used for locomotion. Based on the number and position of flagella there are different types of bacteria (Figure 1.8)
The Gram staining method to differentiate bacteria was developed by Danish Physician Christian Gram in the year 1884. It is a differential staining procedure and it classifies bacteria into two classes - Gram positive and Gram negative. The steps involved in Gram staining procedure is given in Figure 1.10. The Gram positive bacteria retain crystal violet and appear dark violet whereas Gram negative type loose the crystal violet and when counterstained by safranin appear red under a microscope.
Most of the gram positive cell wall contain considerable amount of teichoic acid and teichuronic acid. In addition, they may contain polysaccharide molecules. The gram negative cell wall contains three components that lie outside the peptidoglycan layer. 1. Lipoprotein 2. Outer membrane 3.Lipopolysaccharide. Thus the different results in the gram stain are due to differences in the structure and composition of the cell wall (Figure 1.11). The difference between Gram Positive and Gram negative bacteria is given in Table 1.6.
What are Magnetosomes ?
Intracellular chains of 40-50 magnetite (Fe3O4) particles are found in bacterium Aquaspirillum magnetotacticum. and it help thebacterium to locate nutrient rich sediments.
Two types of respiration is found in Bacteria. They are 1. Aerobic respiration 2. Anaerobic respiration.
These bacteria require oxygen as terminal acceptor and will not grow under anaerobic conditions (i.e. in the absence of O2) Example:Streptococcus.
Some Micrococcus species are obligate aerobes (i.e. they must have oxygen to survive).
These bacteria do not use oxygen for growth and metabolism but obtain their energy from fermentation reactions. Example:Clostridium.
There are bacteria that can grow either using oxygen as a terminal electron acceptor or anaerobically using fermentation reaction to obtain energy. When a facultative anaerobe such as E. coli is present at a site of infection like an abdominal abscess, it can rapidly consume all available O2 and change to anaerobic metabolism producing an anaerobic environment and thus allow the anaerobic bacteria that are present to grow and cause disease.
Example: Escherichia coli and Salmonella.
Bacteria which require CO2 for their growth are called as capnophilic bacteria. Example: Campylobacter.
On the basis of their mode of nutrition bacteria are classified into two types namely Autotrophs and Heterotrophs.
Bacteria which can synthesis their own food are called autotrophic bacteria. They may be further subdivided as
A. Photoautotrophic bacteria
Bacteria use sunlight as their source of energy to synthesize food. They may be
In Photolithotrophs the hydrogen donor is an inorganic substance.
a. Green sulphur bacteria: In this type of bacteria the hydrogen donor is H2S and possess pigment called Bacterioviridin.
b. Purple sulphur bacteria: For bacteria belong to this group the hydrogen donor is Thiosulphate, Bacteriochlorophyll is present.Chlorophyll containing chlorosomes are present Example: Chromatium.
They utilize organic acid or alcohol as hydrogen donor. Example: Purple non sulphur bacteria – Rhodospirillum.
They do not have photosynthetic pigment hence they cannot use sunlight energy. This type of bacteria obtain energy from organic or inorganic substance.
This type of bacteria oxidize inorganic compound to release energy.
1. Sulphur bacteria Thiobacillus thiooxidans
2. Iron bacteria Ferrobacillus ferrooxidans
3. Hydrogen bacteria Hydrogenomonas
4. Nitrifying bacteria Nitrosomonas and Nitrobacter
This type of bacteria oxidize organic compounds to release energy.
1. Methane bacteria – Methanococcus
2. Acetic acid bacteria – Acetobacter
3. Lactic acid bacteria – Lactobacillus
They are Parasites (Clostridium, Mycobacterium) Saprophytes (Bacillus mycoides) or Symbiotic (Rhizobium in root nodules of leguminous crops).
Bacteria reproduces asexually by Binary fission, conidia and endospore formation (Figure 1.12). Among these Binary fission is the most common one.
Under favourable conditions the cell divides into two daughter cells. The nuclear material divides first and it is followed by the formation of a simple median constriction which finally results in the separation of two cells.
During unfavourable condition bacteria produce endospores.
Endospores are produced in Bacillus megaterium, Bacillus sphaericusand Clostridium tetani.
Endospores are thick walled resting spores. During favourable condition, they germinate and form bacteria.
Typical sexual reproduction involving the formation and fusion of gametes is absent in bacteria. However gene recombination can occur in bacteria by three different methods they are
J. Lederberg and Edward L. Tatum demon-strated conjugation in E. coli. in the year 1946. In this method of gene transfer the donor cell gets attached to the recipient cell with the help of pili. The pilus grows in size and forms the conjugation tube. The plas-mid of donor cell which has the F+ (fer-tility factor) undergoes replication. Only one strand of DNA is transferred to the re-cipient cell through conjugation tube. The recipient completes the structure of double stranded DNA by synthesizing the strand that complements the strand acquired from the donor (Figure 1.13).
Transfer of DNA from one bacterium to another is called transformation (Figure 1.14). In 1928 the bacteriologist Frederick Griffith demonstrated transformation in Mice using Diplococcus pneumoniae. Two strains of this bacterium are present. One strain produces smooth colonies and are virulent in nature (S-type). In addition another strain produce rough colonies and are avirulent (R-type). When S-type of cells were injected into the mouse, the mouse died. When R-type of cells were injected, the mouse survived. He injected heat killed S-type cells into the mouse the mouse did not die. When the mixture of heat killed S-type
cells and R-type cells were injected into the mouse. The mouse died. The avirulent rough strain of Diplococcus had been transformed into S-type cells. The hereditary material of heat killed S-type cells had transformed R-type cell into virulent smooth strains. Thus the phenomenon of changing the character of one strain by transferring the DNA of another strain into the former is called Transformation.
Zinder and Lederberg (1952) discovered Transduction in Salmonella typhimurum. Phage mediated DNA transfer is called Transduction (Figure 1.15).
(i) Generalized Transduction (ii) Special-ized or Restricted Transduction
The ability of a bacteriophage to carry genetic material of any region of bacterial DNA is called Generalised transduction.
The ability of the bacteriophage to carry only a specific region of the bacterial DNA is called specialized or restricted transduction.
Bacteria are both beneficial and Harmful. The beneficial activities of bacteria are given in Table 1.7.
Bacteria are known to cause disease in plants, animals and Human beings. The List is given in Table 1.8, 1.9, 1.10 and Figure 1.16.
Collect some root nodules of leguminous crops. Draw diagram. Wash it in tap water and prepare a smear by squeezing the content into a clean slide. Follow Gram staining method and identify the bacteria.
Archaebacteria are primitive prokaryotes and are adapted to thrive in extreme environments like hot springs, high salinity, low pH and so on. They are mostly chemoautotrophs. The unique feature of this group is the presence of lipids like glycerol & isopropyl ethers in their cell membrane. Due to the unique chemical composition the cell membrane show resistance against cell wall antibiotics and lytic agents. Example: Methanobacterium, Halobacterium, Thermoplasma.
Stromatolites reveals the truth.
Stromatolites are deposits formed when colonies of cyanobacteria bind with calcium carbonate. They have a geological age of 2.7 billion years. Their abundance in the fossil record indicates that cyanobacteria helped in raising the level of free oxygen in the atmosphere.
Cyanobacteria are popularly called as 'Blue green algae' or 'Cyanophyceae'. They are photosynthetic, prokaryotic organisms. According to evolutionary record Cyanobacteria are primitive forms and are found in different habitats. Most of them are fresh water and few are marine (Trichodesmium and Dermacarpa) Trichodesmium erythraeum a cyanobacterium imparts red colour to sea (Red sea). Species of Nostoc, Anabaena lead an endophytic life in the coralloid root of Cycas, leaves of aquatic fern Azolla and thallus of hornworts like Anthoceros by establishing a symbiotic association and fix atmospheric nitrogen. Members like Gloeocapsa, Nostoc,Scytonema are found as phycobionts in lichen thalli.
• The members of this group are prokaryotes and lack motile reproductive structures.
• The thallus is unicellular in Chroococcus, Colonial in Gloeocapsa and filamentous trichome in Nostoc.
• Gliding movement is noticed in some species(Oscillatoria).
• The protoplasm is differentiated into central region called centroplasm and peripheral region bearing chromatophore called chromoplasm.
• The photosynthetic pigments include c-phyocyanin and c-phycoerythrin along with myxoxanthin and myxoxanthophyll.
• The reserve food material is Cyanophycean starch.
• The reserve food material is Cyanophycean starch.
• In some forms a large colourless cell is found in the terminal or intercalary position called Heterocysts. They are involved in nitrogen fixation.
• They reproduce only through vegetative methods and produce Akinetes (thick wall dormant cell formed from vegetative cell), Hormogonia (a portion of filament get detached and reproduce by cell division), fission, Endospores.
• The presence of mucilage around the thallus is characteristic feature of this group. Therefore, this group is also called Myxophyceae
• Sexual reproduction is absent.
• Microcystis aeruginosa, Anabaena flos-aquae cause water blooms and release toxins and affect the aquatic organism. Most of them fix atmospheric nitrogen and are used as biofertilizers (Example: Nostoc, Anabaena). Spirulina is rich in protein hence it is used as single cell protein. The thallus organisation and methods of reproduction is given in Figure 1.17
The Mycoplasma are very small (0.1–0.5µm), pleomorphic gram negative microorganisms. They are first isolated by Nocard and co-workers in the year 1898 from pleural fluid of cattle affected with bovine pleuropneumonia. They lack cell wall and appears like “Fried Egg” in culture. The DNA contains low Guanine and Cytosine content than true bacteria. They cause disease in animals and plants. Little leaf of brinjal, witches broom of legumes phyllody of cloves, sandal spike are some plant diseases caused by mycoplasma. Pleuropneumonia is caused by Mycoplasma mycoides. The structure of Mycoplasma is given in Figure 1.18.
Actinomycetes are also called ‘Ray fungi’ due to their mycelia like growth They are anaerobic or facultative anaerobic microorganisms and are Gram positive. They do not produce an aerial mycelium. Their DNA contain high guanine and cytosine content (Example: Streptomyces).
Frankia is a symbiotic actinobacterium which produces root nodules and fixes nitrogen in non – leguminous plants such as Alnus andCasuarina. They produce multicellular sporangium. Actinomyces bovis grows in oral cavities and cause lumpy jaw.
Streptomyces is a mycelial forming Actinobacteria which lives in soil, they impart “earthy odor” to soil after rain which is due to the presence of geosmin (volatile organic compound). Some important antibiotics namely, Streptomycin, Chloramphenicol, and Tetracycline are produced from this genus.