Home | | Biotechnology Applying the Genetic Revolution | Expression of Antisense RNA Constructs

Chapter: Biotechnology Applying the Genetic Revolution: RNA-Based Technologies

| Study Material, Lecturing Notes, Assignment, Reference, Wiki description explanation, brief detail |

Expression of Antisense RNA Constructs

Rather than using antisense oligonucleotides, antisense RNA can be transcribed from a vector that has been inserted into the cell.

EXPRESSION OF ANTISENSE RNA CONSTRUCTS

Rather than using antisense oligonucleotides, antisense RNA can be transcribed from a vector that has been inserted into the cell. First, the target gene is cloned in reverse orientation so that antisense RNA is produced instead of sense mRNA (see Fig. 5.3B). This method is believed to inactivate the cellular target mRNA by forming a heteroduplex of sense/antisense RNA. Heteroduplex formation relies on both RNAs to unfold. If either RNA has a very stable secondary or tertiary structure, then the construct may not work inside the cell.

 

The advantage of internal synthesis of antisense RNA is that the antisense expression can be controlled. If the antisense gene is cloned behind an inducible promoter, then the antisense RNA is not made until the gene is induced by specific signals or conditions. This may be useful to allow organ-specific expression of an antisense gene. Another advantage is that the antisense RNA may be continuously expressed internally over a long-term period. This avoids the inconvenience and expense of constant administration of external antisense oligonucleotides.

A target gene can be cloned in the inverse direction to create an antisense vector. When this is expressed in the cell, the antisense mRNA and endogenous mRNA bind, thus preventing expression into protein.


Study Material, Lecturing Notes, Assignment, Reference, Wiki description explanation, brief detail


Copyright © 2018-2020 BrainKart.com; All Rights Reserved. Developed by Therithal info, Chennai.