Characterization of antibodies

Characterization of antibodies

·     Early physical – chemical studies were done with Ig G from horse, rabbit and human

·     Important structural features were predicted even before sophisti-cated studies were available

·     Molecular weight was calculated from sedimentation and diffusion studies

·     Asymmetrical and or non globular form by viscosity studies

·     Globular domain structure from unique susceptibility to proteolytic enzymes

·     Two antigen binding sites by hapten antibody reactions

·     Thus early studies predicted three functional domains and have been confirmed