Experiment to demonstrate the production of CO2 in aerobic respiration

Experiment to demonstrate the production of CO₂ in aerobic respiration

Take small quantity of any seed (groundnut or bean seeds) and allow them to germinate by imbibing them. While they are germinating place them in a conical flask. A small glass tube containing 4 ml of freshly prepared Potassium hydroxide (KOH) solution is hung into the conical flask with the help of a thread and tightly close the one holed cork (Figure 14.11). Take a bent glass tube, the shorter end of which is inserted into the conical flask through the hole in the cork, while the longer end is dipped in a beaker containing water. Observe the position of initial water level in bent glass tube. This experimental setup is kept for two hours and the seeds were allowed to germinate. After two hours, the level of water rises in the glass tube. It is because, the CO₂ evolved during aerobic respiration by germinating seeds will be absorbed by KOH solution and the level of water will rise in the glass tube.

In the case of groundnut or bean seeds, the rise of water is relatively lesser because these seeds use fat and proteins as respiratory substrate and release a very small amount of CO₂. But in the case of wheat grains, the rise in water level is greater because they use carbohydrate as respiratory substrate. When carbohydrates are used as substrate, equal amounts of CO₂ and O₂ are evolved and consumed.