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Milestones in Plant Tissue Culture
cultured plant cells in artificial condition called in vitro (inside glass) in culture medium (Knop’s salt solution) containing glucose and peptone and developed callus (unorganized growth of cells and tissue) and proposed the concept Totipotency, it means the development of whole plant from isolated cells or tissue in in vitro condition.
developed root cultures, used Knop’s solution along with three vitamins like pyridoxine, thiamine and nicotinic acid
F.C. Steward (1948)
used coconut water in plant tissue culture work and obtained cell proliferation from carrot explants (Cellular totipotency ).
Morel and Martin (1952, 1955)
developed virus-free Dahlia and potato plants using shoot meristem culture.
Murashige and Skoog (1962)
formulated tissue culture medium, a land mark in plant tissue culture and it is the most frequently used medium for all kinds of tissue culture work.
Kanta et al. (1962)
produced test-tube fertilization in flowering plants.
Yamada et al. (1963)
produced calli and free cells in tissue culture of Tradescantia reflexa.
Guha and Maheshwari (1964)
developed in vitro production of haploid embryos from anthers of Datura.
Vasil and Hildbrandt (1965)
achieved differentiation of tobacco plants from single, isolated cells in micro propagation.
Takebe et al. (1971)
regenerated tobacco plants from isolated mesophyll protoplasts.
and co-workers obtained protoplast fusion between Nicotiana glauca and Nicotiana longsdorffii and developed first interspecific somatic hybrid in 1971.
Melchers and co-workers in 1978
developed intergenic hybrid between potato and tomato called pomato.
produced transformed tobacco plants from single cell transformation and gene insertion.
Horsh et al. (1984)
developed transgenic tobacco by Agrobacterium mediated gene transfer.
Knop’s solution: Nutrient solution used in growth experiments of plants which contains:
Calcium nitrate 3.0 g
Potassium nitrate 1.0 g
Sucrose 50.0 g (optimal)
Magnesium sulfate 1.0 g
Dibasic Potassium phosphate 1.0 g
Deionized water 1000.0 ml
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