Basic concepts of Tissue Culture
Basic concepts of plant tissue culture are totipotency,
differentiation, dedifferentiation and redifferentiation.
The property of live plant cells that they have the genetic
potential when cultured in nutrient medium to give rise to a complete
The process of biochemical and structural changes by which cells become specialized in form and function.
The further differentiation of already differentiated cell into
another type of cell. For example, when the component cells of callus have the
ability to form a whole plant in a nutrient medium, the phenomenon is called
The phenomenon of the reversion of mature cells to the
meristematic state leading to the formation of callus is called
dedifferentiation. These two phenomena of redifferentiation and
dedifferentiation are the inherent capacities of living plant cells or tissue.
This is described as totipotency.