Aseptic condition: Preparation of materials free from microbes in in vitro cultures.
Cell Culture: Growing of cells in vitro, including the culture of single cells or small aggregates of cells in a liquid medium.
Chemically defined medium : A nutritive medium used for culturing cells or tissue; each chemical of this medium is known and defined;
Cybrid: Cytoplasmic hybrid obtained by the fusion of cytoplasm of cells of different parental sources; a term applied to the fusion of cytoplasms of two different protoplasts;
Organogenesis: The process of initiation and development of shoot or root though in vitro culture particularly from callus