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Chapter: Genetics and Molecular Biology: Genetic Engineering and Recombinant DNA

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Biology of Restriction Enzymes

In this section we digress into the biology of restriction enzymes and then return to their use in cutting DNA.

The Biology of Restriction Enzymes

In this section we digress into the biology of restriction enzymes and then return to their use in cutting DNA. A large number of enzymes have now been found that cut DNA at specific sites. For the most part the enzymes come from bacteria. These enzymes are called restriction enzymes because in the few cases that have been carefully studied, the DNA cleaving enzyme is part of the cell’s restriction-modification sys-tem.

The phenomenon of restriction-modification in bacteria is a small-scale immune system for protection from infection by foreign DNA. In contrast to higher organisms in which identification and inactivation of invading parasites, bacteria, or viruses can be performed extracellularly, bacteria can protect themselves only after foreign DNA has entered their cytoplasm. For this protection, many bacteria specifically mark their own DNA by methylating bases on particular sequences with modifying enzymes. DNA that is recognized as foreign by its lack of methyl groups on these same sequences is cleaved by the restriction enzymes and then degraded by exonucleases to nucleotides. Less than one phage out of 104 wrongly methylated infecting phage is able to grow and lyse an E. coli protected by some restriction-modification systems. Bacteria fur-ther protect themselves from plant and animal DNA. Much plant and animal DNA is methylated on the cytosine in CpG sequences. Many strains of bacteria also contain enzymes that cleave DNA when it is methylated on specific positions.

Arber studied restriction of lambda phage in E. coli and found that E. coli strain C does not contain a restriction-modification system.Strain B has one restriction-modification system, and yet a different one recognizes and methylates a different nucleotide sequence in strain K-12. Phage P1 also specifies a restriction-modification system of its own, and this can be superimposed on the restriction-modification system of a host in which it is a lysogen.

Table 9.1 Plating Efficiencies of Phage Grown onE.coli C, K, and B and Plated on These Bacteria


 


Let the notation λ-C represent lambda phage that has been grown on E. coli strain C. Infection of strains B, K-12, and C withλgrown on thevarious strains yields different efficiencies of plaque formation (Table 9.1). Passage of the phage through a host of one type modifies the DNA so that it is recognized as “self” and plates at high efficiency if the phage reinfects that same strain. It is recognized as “foreign” and plates at low efficiency if the phage infects a strain with a different restriction-modification system.


Figure 9.2 Methylation of an asymmetrical sequence necessitates recognitionand methylation of two different sequences on the daughter strands on DNA replication.


Possession of a restriction-modification system introduces complexities to the process of DNA replication. Imagine that the double-stranded DNA contains methyl groups on both strands of the DNA at a recognition sequence. DNA replication creates a new duplex in which one of the strands in each of the daughter duplexes at first lacks the modification. This half-methylated DNA must not be recognized as foreign DNA and cleaved, but instead must be recognized as “self” and methylated (Fig. 9.2). Therefore, the restriction-modification system functions like a microcomputer, recognizing three different states of methylation of its recognition sequence and taking one of three different actions. If the sequence is unmethylated, the enzymes cleave. If the DNA is methylated on one of the two strands, the modification system methylates the other strand; if the DNA is methylated on both strands, the enzymes do nothing.

A palindromic recognition sequence streamlines operation of the restriction-modification system. A palindrome is a sequence that reads the same forward and backward, such as repaper and radar. Because DNA strands possess a direction, we consider a DNA sequence to be palindromic if it is identical when read 5’ to 3’ on the top strand and on the bottom strand (Fig. 9.3). Palindromes, of course, can be of any size, but most that are utilized as restriction-modification recognition se-quences are four, five, six, and rarely, eight bases long. By virtue of the properties of palindromes, both daughter duplexes of replicated palin-dromic sequences are identical, and thus the modification enzyme needs to recognize and methylate only one type of substrate (Fig. 9.4). As we already saw in Fig. 9.2, the use of nonpalindromic recognition sequences would require that the modification enzyme recognize two different sequences. Presumably dimeric proteins are used to recognize the palindromic sequences.



 

Restriction enzymes are divided into three main classes. The enzymes in class I form a complex consisting of a cleaving subunit, a methylating subunit, and a sequence recognition subunit. These enzymes cleave at sites far distant from their recognition sequences and will not be further discussed here even though they were the first to be discovered. Those in class II possess their sequence recognition and cleaving activities together. They cleave in or near their recognition sequence and are of the most use in genetic engineering. The class III enzymes possess a cleavage subunit associated with a recognition and methylation subunit. These cleave near their recognition site.

A restriction enzyme within a cell is a time bomb because physical-chemical principles limit the enzyme’s specificity for binding. If a restriction enzyme were to bind to a wrong sequence, and a typical bacterium contains about 4 × 106 such sequences, most likely the sequence would not be methylated and the enzyme could cleave. This would break the chromosome, and the cell would die. The experimental observation, however, is that cells containing restriction enzymes do not noticeably die any faster than cells without restriction enzymes. How, then, is the extraordinarily high specificity of the restriction enzymes generated?

Figure 9.4 Methylation of a palindromic sequence permits recognition andmethylation of only one sequence during DNA replication.



The requisite high specificity can be obtained if cutting the DNA duplex is a two-step process. An enzyme could bind to the recognition sequence, cleave one strand, wait a while, then cleave the other strand. This has the effect of utilizing the recognition sequence twice for each cleavage. If the enzyme binds at a site other than the recognition sequence, it rapidly dissociates before cleaving the second strand. Therefore, restriction enzymes are likely to produce nicks in the DNA at sites other than the recognition sequence, but these nicks can be repaired with DNA ligase and the cell will not be harmed in the process. Few restriction enzymes are likely to be found that cleave both strands of the DNA at an incorrect site in a concerted process.


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