Home | | Genetics and Molecular Biology | Arrest of Translation to Assay for DNA of a Gene

Chapter: Genetics and Molecular Biology: Genetic Engineering and Recombinant DNA

Arrest of Translation to Assay for DNA of a Gene

In vitro translation of mRNA forms the basis of one technique foridentification of clones containing DNA of a particular gene.

Arrest of Translation to Assay for DNA of a Gene

In vitro translation of mRNA forms the basis of one technique foridentification of clones containing DNA of a particular gene. The technique requires that the enriched messenger RNA of the gene, such as the mRNA obtained from an oligo-dT column, be translatable in vitro to yield a detectable protein product of the desired gene. To perform the identification, DNA for a candidate clone is denatured and hybridized to the RNA used in the translation mixture. If the DNA contains sequences complementary to the mRNA, the two will hybridize to-gether, the messenger will become unavailable for in vitro translation, and the gene product will not be synthesized (Fig. 9.15). DNA from a clone not possessing the DNA sequence of the gene will not interfere

Figure 9.15 Hybridization arrest of translation as an assay for DNA of a genewhose mRNA can be translated into protein.



with translation of the messenger. Hence DNA carrying sequences complementary to the messenger can be detected by hybridization arrest of translation.


Study Material, Lecturing Notes, Assignment, Reference, Wiki description explanation, brief detail
Genetics and Molecular Biology: Genetic Engineering and Recombinant DNA : Arrest of Translation to Assay for DNA of a Gene |


Privacy Policy, Terms and Conditions, DMCA Policy and Compliant

Copyright © 2018-2024 BrainKart.com; All Rights Reserved. Developed by Therithal info, Chennai.