Home | | Genetics and Molecular Biology | Testing Purified Cultures, Scoring

Chapter: Genetics and Molecular Biology: Genetics

| Study Material, Lecturing Notes, Assignment, Reference, Wiki description explanation, brief detail |

Testing Purified Cultures, Scoring

Once a strain has been purified, its phenotype can be tested. Suppose, for example, that a desired bacterial strain is claimed to be unable to use lactose as a sole source of carbon and to be unable to synthesize arginine.

Testing Purified Cultures, Scoring

Once a strain has been purified, its phenotype can be tested. Suppose, for example, that a desired bacterial strain is claimed to be unable to use lactose as a sole source of carbon and to be unable to synthesize arginine. What is the simplest way to test or score for Lac- Arg-? One satisfactory growth medium for the mutant would be a medium con-taining K+, Na+, PO4- -, SO4- -, Mg++, Ca++, Fe++, NH4+, and other trace elements which are present as chemical contaminants of these ingredi-ents, plus glucose at a concentration of 2 gm/liter and L-arginine at 0.1 gm/liter. This is called a minimal salts medium with glucose and arginine. Each candidate colony could be spotted with sterile wooden sticks or toothpicks onto minimal salts medium containing the follow - ing additions: glucose, glucose plus arginine, and lactose plus arginine. The desired strain should grow only on glucose plus arginine medium (Table 8.1).


 


Study Material, Lecturing Notes, Assignment, Reference, Wiki description explanation, brief detail


Copyright © 2018-2020 BrainKart.com; All Rights Reserved. Developed by Therithal info, Chennai.