Lipid Catabolism
Microorganisms
frequently use lipids such as triglyceride or triacylglycerol (esters of
glycerol and fatty acids) as common reserve energy sources. These can be
hydrolyzed to glycerol and fatty acid by microbial lipases. The glycerol is
then phosphorylated and oxidized to Dihydroxyacetone phosphate and then
catabolized in the Glycolysis pathway. Fatty acids from triacylglycerols and
other lipids are often oxidized in the β-oxidation pathway. In this pathway
fatty acids are degraded to acetyl CoA (2C segment), then it enters into the
TCA cycle
Bubble Test: Bubbles are a positive result for the presence of catalase. If
an organism can produce catalase, it will produce bubbles of oxygen when
hydrogen peroxide is added to it.
Infobits
One of the major environmental problems today is hydrocarbon
contamination resulting from the activities related to the petrochemical industry.
Several bacteria can use hydrocarbon as a feed and reduce pollution.
Pseudomonas putida
(Super Bug) Alcanivorax borkumens,Mycobacterium, Brevibacterium,
Aspergillus, Penicillium, Candida lipolytica are the most active agents in petroleum degradation and they work as
primary degraders in oil spilled environment. These organisms are mainly in
involved in bioremediation which reduce environmental pollution
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