Microorganisms frequently use lipids such as triglyceride or triacylglycerol (esters of glycerol and fatty acids) as common reserve energy sources. These can be hydrolyzed to glycerol and fatty acid by microbial lipases. The glycerol is then phosphorylated and oxidized to Dihydroxyacetone phosphate and then catabolized in the Glycolysis pathway. Fatty acids from triacylglycerols and other lipids are often oxidized in the β-oxidation pathway. In this pathway fatty acids are degraded to acetyl CoA (2C segment), then it enters into the TCA cycle
Bubble Test: Bubbles are a positive result for the presence of catalase. If an organism can produce catalase, it will produce bubbles of oxygen when hydrogen peroxide is added to it.
One of the major environmental problems today is hydrocarbon contamination resulting from the activities related to the petrochemical industry. Several bacteria can use hydrocarbon as a feed and reduce pollution.
Pseudomonas putida (Super Bug) Alcanivorax borkumens,Mycobacterium, Brevibacterium, Aspergillus, Penicillium, Candida lipolytica are the most active agents in petroleum degradation and they work as primary degraders in oil spilled environment. These organisms are mainly in involved in bioremediation which reduce environmental pollution