Chapter: Biochemistry: Biochemical Techniques

Applications of spectrophotometry

Colorimetry and spectrophotometry have widest application in biological sciences.

Applications of spectrophotometry

Colorimetry and spectrophotometry have widest application in biological sciences. These techniques are used for the determination of

· glucose, proteins, lipids, nucleic acid etc

· turbidity of solutions( bacterial cell mass)

· absorption spectrum of a compound

· purity of compound by knowing the molar extinction coefficient which is maximum for a pure compound.

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Biochemistry: Biochemical Techniques : Applications of spectrophotometry |