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Chapter: Biochemistry: Biochemical Techniques

Applications of chromatography

Thin layer chromatography is used for the separation of alkaloids, phospholipids and other lipids.

Applications of chromatography

·           Thin layer chromatography is used for the separation of alkaloids, phospholipids and other lipids.

·           Gas liquid chromatography is applied for the separation of fatty acids in a lipid mixture.

·           Ion exchange chromatography can be used for the separation and identification of amino acids in a mixture of protein hydrolysate. This principle is used in auto-analyzer.

·           Exclusion chromatography can be applied for the determination of the molecular weight of the components separated.

·           Affinity chromatography is applied for the purification of a wide range of enzymes and other proteins like immunoglobulins.

 

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Biochemistry: Biochemical Techniques : Applications of chromatography |


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