1. The first clinical gene therapy was done for the treatment of
d) Cystic fibrosis
2. Dolly, the sheep was obtained by a technique known as
a) Cloning by gene transfer
b) Cloning without the help of gametes
c) Cloning by tissue culture of somatic cells
d) Cloning by nuclear transfer
3. The genetic defect adenosine deaminase deficiency may be cured permanently by
a) Enzyme replacement therapy
b) periodic infusion of genetically engineered lymphocytes having ADA cDNA
c) administering adenosine deaminase activators
d) introducing bone marrow cells producing ADA into embryo at an early stage of development.
4. GEAC stands for
a) Genome Engineering Action Committee
b) Ground Environment Action Committee
d) Genetic and Environment Approval Committee
5. How many amino acids are arranged in the two chains of Insulin?
a) Chain A has 12 and Chain B has 13
b) Chain A has 21 and Chain B has 30 amino acids
c) Chain A has 20 and chain B has 30 amino acids
d) Chain A has 12 and chain B has 20 amino acids.
6. PCR proceeds in three distinct steps governed by temperature, they are in order of
a) Denaturation, Annealing, Synthesis
b) Synthesis, Annealing, Denaturation
c) Annealing, Synthesis, Denaturation
d) Denaturation, Synthesis, Annealing
7. Which one of the following statements is true regarding DNA polymerase used in PCR?
a) It is used to ligate introduced DNA in recipient cells
b) It serves as a selectable marker
c) It is isolated from a Virus
d) It remains active at a high temperature.
8. ELISA is mainly used for
a) Detection of mutations
b) Detection of pathogens
c) Selecting animals having desired traits
d) Selecting plants having desired traits
9. Transgenic animals are those which have
a) Foreign DNA in some of their cells
b) Foreign DNA in all their cells
c) Foreign RNA in some of their cells
d) Foreign RNA in all their cells
10. Recombinant Factor VIII is produced in the ------- cells of the Chinese Hamster
a) Liver cells
b) blood cells
c) ovarian cells
d) brain cells.
11.Vaccines that use components of a pathogenic organism rather than the whole organism are called
a) Subunit recombinant vaccines
b) attenuated recombinant vaccines
c) DNA vaccines
d) conventional vaccines.
12. Mention the number of primers required in each cycle of PCR. Write the role of primers and DNA polymerase in PCR. Name the source organism of the DNA polymerase used in PCR.
13. How is the amplification of a gene sample of interest carried out using PCR?
14. What is genetically engineered Insulin?
15. Explain how “Rosie” is different from a normal cow.
16. How was Insulin obtained before the advent of rDNA technology? What were the problems encountered?
17. ELISA is a technique based on the principles of antigen-antibody reactions. Can this technique be used in the molecular diagnosis of a genetic disorder such as Phenylketonuria?
18. Gene therapy is an attempt to correct a Genetic defect by providing a normal gene into the individual. By this the function can be restored. An alternate method would be to provide gene product known as enzyme replacement therapy, which would also restore the function. Which in your opinion is a better option? Give reasons for your answer.
19. What are transgenic animals? Give examples.
20. If a person thinks he is infected with HIV, due to unprotected sex, and goes for a blood test. Do you think a test such as ELISA will help? If so why? If not, why?
21. Explain how ADA deficiency can be corrected?
22. What are DNA vaccines?
23. Differentiate between Somatic cell gene therapy and germline gene therapy.
24. What are stem cells? Explain its role in the field of medicine.
25. What are the possible risks of GMOs?
26. One of the applications of biotechnology is ‘gene therapy” to treat a person born with a hereditary disease.
i. What does “gene therapy” mean?
ii. Name the hereditary disease for which the first clinical gene therapy was used.
iii. Mention the steps involved in gene therapy to treat this disease.
27. PCR is a useful tool for early diagnosis of an Infectious disease. Elaborate.
28. What are recombinant vaccines?. Explain the types.
29. Explain why cloning of Dolly, the sheep was such a major scientific breakthrough?
30. Mention the advantages and disadvantages of cloning.
31. Explain how recombinant Insulin can be produced.
32. Explain the steps involved in the production of recombinant hGH.
Extra One mark Questions and Answers
1. Genetic engineering has been successfully used for producing
a) Transgenic mice for testing safety of polio vaccine before used in humans.
b) Transgenic models for studying new treatments for certain cardiac diseases.
c) Transgenic cow Rosie which produces high fat milk for making ghee.
d) Animals like bulls for farm work as they have super power.
2. Some of the characteristics of Bt cotton are
a) Long fibre and resistance to aphids
b) Medium yield, long fibre and resistance to beetle pests.
c) High yields and production of toxic protein crystals which kill dipteran pests.
d) High yield and resistance to bollworms
3. Bacillus thuringiensis forms protein crystals which contain insecticidal protein. This protein
a) Binds with epithelia cells of midgut of the insect pest ultimately killing it.
b) Is coded by several genes including the gene cry.
c) Is activated by acid pH of the foregut of the insect pest.
d) Does not kill the carrier bacterium which is itself resistant to this toxin.
4. Read the following four statements (A to D) about certain mistakes in two of them.
A) The first transgenic buffalo, Rosie produced milk which was human alpha – lactalbumin enriched.
B) Restriction enzymes are used in isolation of DNA from other macromolecules.
C) Downstream processing is one of the steps of rDNA technology
D) Disarmed pathogen vectors are also used in transfer of rDNA into the host.
Which of the two statements have mistakes?
a) B and C
b) C and D
c) A and C
d) A and B
5. The colonies of recombinant bacteria appear white in contrast to blue colonies of non-recombinant bacteria because of
a) Non-recombinant bacteria containing β-galactosidase.
b) Insertional inactivation of α-galactosidase in non-recombinant bacteria.
c) Insertional inactivation of α-galactosidase in recombinant bacteria.
d) Inactivation of glycosidase enzyme in recombinant bacteria
6. Which body of the Government of India regulates GM research and safety of introducing GM organism for public services?
a) Bio-safety committee
b) Indian council of agricultural research
c) Genetic engineering approval committee
d) Research committee on Genetic manipulation
7. In genetic engineering, a DNS segment (gene) of interest is transferred to the host cell through a vector. Consider the following four agents (A to D) in this regard and select correct option about which one or more of these can be used as vector/vectors.
A) A bacterium B) Plasmid
C) Plasmodium D) Bacteriophage
a) (A), (B) and (D) only
b) (A) only
c) (A) and (C) only
d) (B) and (D) only
8. Which one of the following palindromic base sequences in DNA can be easily cut at about the middle by some particular restriction enzyme?
a) 5’ – CGTTCG – 3’ 3’ –ATGGTA -5’
b) 5’-GATATG -3’ 3’ CTACTA -5’
c) 5’ –GAATTC – 3’ 3’ – CTTAAG-5’
d) 5’ –CACGTA -3’ 3’ –CTCAGT -5’
9. Restriction endonucleases are enzymes which
a) Make cuts at specific positions within the DNA molecule.
b) Recognize a specific nucleotide sequence for binding of DNA ligase.
c) Restrict the action of the enzyme DNA polymerase.
d) Remove nucleotides from the ends of the DNA molecule.
10. Stirred – tank bioreactors have been designed for
a) Addition of preservatives of the product
b) Purification of the product
c) Ensuring anaerobic conditions in the culture vessel
d) Availability of oxygen throughout the process
11. There is a retriction endonuclease called EcoRI. What does ‘co’ part in it stand for?
12. Which one is true state regarding DNA polymerase used in PCR?
a) It is used to ligate introduced DNA in recipient cells.
b) It serves as selectable marker
c) It is isolated from a virus.
d) It remains active at high temperature.
13. For transformation, micro-particles coated with DNA to be bombarded with gene gun are made up of
a) Silver or Platinum
b) Platinum or Zinc
c) Silicon or Platinum
d) Gold or Tungsten
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