Plant tissue culture - Callus with plantlets

Models / Photographs / Charts

Exercise: Plant tissue culture – Callus with plantlets

Aim: To study and identify the Callus with plantlets.

Principle: Growing the plant cells, tissues and organs in an artificial, synthetic medium under controlled conditions is called plant tissue culture. The technique of cloning plant is easier than animals because plant cells are simple in structure and most plant cells shows totipotency (i.e) ability to regenerate from cells.

Requirements: Model / Photograph / Picture of callus with plantlets.

Diagnostic Features

·        The callus is an unorganized mass of undifferentiated tissue.

·        The mechanism of callus formation is that auxin induce cell elongation and cytokinin induces cell division as a result of which masses of cells are formed.

·        Roots and shoots are differentiated from the callus.