Models / Photographs / Pictures | Botany Practicals - E.coli cloning vector (pBR 322) | 12th Botany : Practicals
Chapter: 12th Botany : Practicals
E.coli cloning vector (pBR 322)
Models / Photographs / Pictures
Exercise: E.coli cloning vector (pBR 322)
Aim: To study and identify
the features of cloning vector – pBR 322
Principle: Vectors are used as carriers
to deliver the desired foreign DNA into a host cell.
Requirements: Models/ Photographs /
Pictures of E.coli Cloning vector pBR 322.
Diagnostic Features
·
pBR 322 plasmid is a reconstructed plasmid containing 4361 base
pairs and most widely used as cloning vector.
·
In pBR, p denotes plasmid and B and R respectively the notes of
scientists Boliver and Rodriguez who developed the plasmid. The number 322 is
the number of plasmids developed from their laboratory.
·
It contains two different antibiotic resistance genes and
recognition site for several restriction enzymes (Hind III, Eco R I, Bam H I,
Sal I, Pvu II, Pst I, Cla I), Ori and antibiotic resistance genes (ampR and
tetR). Rop codes for the proteins involved in the replication of the plasmid.
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