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Chapter: 12th Botany : Practicals

Analysis of seed sample to study Mendelian dihybrid ratio

Aim: To analyse seed sample of pea for Mendelian dihybrid ratio of 9 : 3 : 3 : 1.

Exercise: Analysis of seed sample to study Mendelian dihybrid ratio

Aim:

To analyse seed sample of pea for Mendelian dihybrid ratio of 9 : 3 : 3 : 1.

Principle:

In a dihyrbid cross, the segregation of one gene pair is independent of the segregation of the other pair. It means that when the factors (genes) for different characters inherited from parents do not remain linked in the offsprings, but their distribution in the gametes and in the progeny of subsequent generations is independent of each other.

Requirement:

Plastic beakers, Pea seed samples or plastic beads, tray, petri dishes, notebook, pencil / pen.

Teachers should select the Pea seed or plastic beads which represents the four types of traits such as yellow round, yellow wrinkled, green round and green wrinkled in the ratio of 9:3:3:1

Procedure:

¬∑        Take a lot of about 160 Pea seeds or plastic beads in a tray.

¬∑        Separate out yellow round, yellow wrinkled, green round and green wrinkled and put them in separate petridishes.

¬∑        Note down the number of seeds in each plate and find out their approximate ratio.


Observation:

Present your finding in the form of a table.


Inference:

The ratio of yellow round : yellow wrinkled : Green round : green wrinkled is approximately 9 : 3 : 3 : 1  which is exactly the same as obtained by Mendel for a dihybrid cross. This indicates that the contrasting genes for seed colour and seed shape show an independent assortment in the population of pea seeds. 


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