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Chapter: Plant Biochemistry: Photosynthesis is an electron transport process

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Photosystem I reduces NADP

Plastocyanin that has been reduced by the cyt-b6/f complex diffuses through the lumen of the thylakoids, binds to a positively charged binding site of PS I, transfers its electron, and the resulting oxidized form diffuses back to the cyt-b6/f complex .

Photosystem I reduces NADP

 

Plastocyanin that has been reduced by the cyt-b6/f complex diffuses through the lumen of the thylakoids, binds to a positively charged binding site of PS I, transfers its electron, and the resulting oxidized form diffuses back to the cyt-b6/f complex (Fig. 3.31).


 

Also the reaction center of PS I with an absorption maximum of 700 nm contains a chlorophyll pair (chl-a)2 (Fig. 3.31). As in PS II, the excitation caused by a photon reacts probably with only one of the two chlorophyll molecules. The resulting (chl-a)2+  is then reduced by plastocyanin. It is assumed that (chl-a)2 transfers its electron to a chl-a monomer (A0), which then transfers the electron to a strongly bound phylloquinone (Q) (Fig. 3.32). Phylloquinone contains the same phytol side chain as chl-a and its function corresponds to QA in PS II. The electron is transferred from the semiphylloquinone to an iron-sulfur center named FX. FX is a 4Fe-4S center with a very negative redox potential. It transfers one electron to two other 4Fe-4S centers (FA, FB), which in turn reduce ferredoxin, a protein with a molecular mass of 11 kDa with a 2Fe-2S center. Ferredoxin also takes up and transfers only one electron. The reduction occurs at the stromal side of the thylakoid membrane. For this purpose, the ferredoxin binds at a posi-tively charged binding site on subunit D of PS I (Fig. 3.33). The reduction of NADP+ by ferredoxin, catalyzed by ferredoxin-NADP reductase, yields NADPH as an end product of the photosynthetic electron transport.


 


The PS I complex consists of at least 17 different subunits, of which some are shown in Table 3.4. The center of the PS I complex is a heterodimer (as is the center of PS II) consisting of subunits A and B (Fig. 3.33). The molec-ular masses of A and B (each 82–83 kDa) correspond approximately to the sum of the molecular masses of the PS II subunits D1 and CP43, and D2 and CP47, respectively (Table 3.2). In fact, both subunits A and B have a dou-ble function. Like D1 and D2 in PS II, they bind chromophores (chl-a) and redox carriers (phylloquinone, FeX) of the reaction center and, additionally, they contain about 100 chl-a molecules as antennae pigments. Thus, the het-erodimer of A and B represents the reaction center and the core antenna as well. The three-dimensional structure of photosystem I in cyanobacteria, green algae and plants has been resolved. The principal structure of pho-tosystem I, with a central pair of chl-a molecules and two branches, each with two chlorophyll molecules, is very similar to photosystem II and to the bacterial photosystem (Fig. 3.10). It has not been definitely clarified whether both or just one of these branches are involved in the electron transport. The Fe-S-centers FA and FB are ascribed to subunit C, and subunit F is con-sidered to be the binding site for plastocyanin.

 



 


The light energy driving the cyclic electron transport of PS I is only utilized for the synthesis of ATP

 

Besides the noncyclic electron transport discussed so far, cyclic electron transfer can also take place in which the electrons from the excited pho-tosystem I are transferred back to the ground state of PS I, probably via the cyt-b6/f complex (Fig. 3.34). The energy thus released is used only for the synthesis of ATP, and NADPH is not formed. This electron transport is termed cyclic photophosphorylation. In intact leaves, and even in iso-lated intact chloroplasts, it is quite difficult to differentiate experimentally between cyclic and non-cyclic photophosphorylation. It has been a matter of debate as to whether and to what extent cyclic photophosphorylation occurs in a leaf under normal physiological conditions. Recent evaluations of the proton stoichiometry of photophosphorylation  sug-gest that the yield of ATP in noncyclic electron transport is not sufficient for the requirements of CO2 assimilation, and therefore cyclic photophos-phorylation seems to be required to synthesize the lacking ATP. Moreover, cyclic photophosphorylation must operate at very high rates in the bundle sheath chloroplasts of certain C4 plants . These cells have a high demand for ATP and they contain high PS I activity but very little PS II. Presumably, the cyclic electron flow is governed by the redox state of the acceptor of the photosystem in such a way that by increasing the reduction of the NADP system, and consequently of ferredoxin, the diversion of the electrons in the cycle is enhanced. The function of cyclic electron transport is probably to adjust the rates of ATP and NADPH formation according to the plant’s demand.

 

Despite intensive investigations, the pathway of electron flow from PS I to the cyt-b6/f complex in cyclic electron transport remains unresolved. It has been proposed that cyclic electron transport is structurally separated from the linear electron transport chain in a super complex. Most experi-ments on cyclic electron transport have been carried out with isolated thy-lakoid membranes that catalyze only cyclic electron transport when redox mediators, such as ferredoxin or flavin adenine mononucleotide (FMN, Fig. 5.16), have been added. Cyclic electron transport is inhibited by the antibiotic antimycin A. It is not clear at which site this inhibitor functions. Antimycin A does not inhibit noncyclic electron transport.

 

Surprisingly, proteins of the NADP dehydrogenase complex of the mitochondrial respiratory chain  have also been identified in the thylakoid membrane of chloroplasts. The function of these proteins in chloroplasts is still not known. The proteins of this complex occur very fre-quently in chloroplasts from bundle sheath cells of C4 plants, which have little PS II but a particularly high cyclic photophosphorylation activity . These observations raise the possibility that in cyclic electron transport the flow of electrons from NADPH or ferredoxin to plastoqui-none proceeds via a complex similar to the mitochondrial NADH dehydro-genase complex. The mitochondrial NADH dehydrogenase complex transfers electrons from NADH to ubiquinone. Results indicate that an additional pathway for a cyclic electron transport exists in which electrons are directly transferred via a plastoquinone reduct-ase from ferredoxin to plastoquinone.


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