Immunoelectronmicroscopic Tests - Antigen Antibody Reactions

Immunoelectronmicroscopic Tests

These are the types of antigen–antibody reactions that are visualized directly by electron microscope. These are of the following types:

◗ Immunoelectronmicroscopy

This is a test used to detect rotavirus and hepatitis A virus directly in feces. In this test, viral particles are mixed with spe-cific antisera and are demonstrated as clumps of virion particles under the electron microscope.

◗ Immunoenzyme test

This test is used to detect antigen directly in tissue specimens, in which tissue sections are treated with peroxidase-labeled antisera to detect corresponding antigen. The peroxidase bound to the antigen is visualized under the electron microscope.

◗ Immunoferritin test

Electron-dense substances, such as ferritin are conjugated with antibody and such labeled antibodies reacting with antigen can be visualized under the electron microscope.