Chapter: Biotechnology Applying the Genetic Revolution: Recombinant Proteins
Comparing Expression Systems
Each expression system has its own unique strengths and weaknesses when it comes to characteristics such as cost, speed, and ability to glycosylate or fold proteins, as well as government regulations.
COMPARING
EXPRESSION SYSTEMS
Each expression system has
its own unique strengths and weaknesses when it comes to characteristics such
as cost, speed, and ability to glycosylate or fold proteins, as well as
government regulations. Bacterial cells are by far the cheapest and fastest
method for recombinant protein expression, but they do not glycosylate proteins
or fold mammalian proteins correctly. Cultured mammalian cells glycosylate and
fold proteins properly, but they are expensive to maintain. Therefore,
determining what system to use to express recombinant proteins depends on the
protein and its particular idiosyncrasies (Fig. 10.15).
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Biotechnology Applying the Genetic Revolution: Recombinant Proteins : Comparing Expression Systems |
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Biotechnology Applying the Genetic Revolution: Recombinant Proteins