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Chapter: Pharmaceutical Drug Analysis: Thin Layer Chromatography (TLC)

Purification of Silica Gel-G Layers - Thin Layer Chromatography (TLC)

The iron present as an impurity in silica gel-G affords an appreciable distortion of the ‘chromatogram’.

PURIFICATION OF SILICA GEL-G LAYERS

 

The iron present as an impurity in silica gel-G affords an appreciable distortion of the ‘chromatogram’. Hence, it has become almost a necessary step to purify the adsorbent. The ‘iron-free’ layers may be achieved by providing the pre-coated and air-dried plates a preliminary development with a mixture of methanol and concentrated hydrochloric acid* (9 : 1). By this process the entire iron gets migrated with the solvent front to the upper boundary of the TLC plate. Consequently, the purified plates are again dried and activated at 110°C.

 

The cleaning process usually washes out the CaSO4 originally present as binder. Therefore, the silica gel thus obtained by purification may be reused to prepare TLC-plates with other appropriate binders like gypsum, starch etc.

 

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Pharmaceutical Drug Analysis: Thin Layer Chromatography (TLC) : Purification of Silica Gel-G Layers - Thin Layer Chromatography (TLC) |


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