COMBINATION OF TLC WITH OTHER TECHNIQUES
TLC may be combined with column chromatography and vapour-phase
chromatography as discussed here briefly :
TLC helps in
selecting the best combination of solvent and adsorbent for a given column
separation. Miller and Kirchner**** (1952) developed this combination
thoroughly and employed it extensively for the separation of a large number of
difficult types of compounds.
The
various spots obtained from TLC may be eluted, concentrated and then subjected
to vapour-phase chromatogrphy studies. Ikeda et al***** (1961, 1962) exploited this combination for the analysis
of a variety of naturally occurring constituents, namely :
(i) Citrus oils
and other essential oils,
(ii) Oestrogens
in urine sample,
(iii)
Testosterone in urine sample, and
(iv)
Progesterone in plasma.
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