COMBINATION OF TLC WITH OTHER TECHNIQUES
TLC may be combined with column chromatography and vapour-phase chromatography as discussed here briefly :
TLC helps in selecting the best combination of solvent and adsorbent for a given column separation. Miller and Kirchner**** (1952) developed this combination thoroughly and employed it extensively for the separation of a large number of difficult types of compounds.
The various spots obtained from TLC may be eluted, concentrated and then subjected to vapour-phase chromatogrphy studies. Ikeda et al***** (1961, 1962) exploited this combination for the analysis of a variety of naturally occurring constituents, namely :
(i) Citrus oils and other essential oils,
(ii) Oestrogens in urine sample,
(iii) Testosterone in urine sample, and
(iv) Progesterone in plasma.