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Chapter: Medical Electronics - Bio-Chemical and Non Electrical Parameter Measurement

Colorimeter

Measures the color concentration of a substance in a solution by deteecting the color light intensity passing through a sample containing the substance and a reagent

COLORIMETER

 

·        Measures the color concentration of a substance in a solution by deteecting the color light intensity passing through a sample containing the substance and a reagent

 

·        Optical color filters are used to detect the color wavelength of interest. E.g., urine passes yellow light and absorbs blue and green

 

·        Laser LEDs are preferred if their wavelength is suitable due to purity of the monochromatic color.

 


Transmittance

 

T= I1/I0 * 100%

 

Absorbance

 

A=  - log I1/ I0

 

A=log 1/T

 

If the path length or concentration increases, the transmittance decreas es and absorbance increases, a phenomenon expres sed by Beer’s Law.

 

Absorbtivity related to the nature of the A=aCL absorbing substance and optical wavelength (known for a standar d solutionconcentration).

 

C: Concentration

L: Cuvette path length

 

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