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Chapter: 11th Biochemistry : Basic Concepts of Bio Chemistry and Cell Biology

Uses of Buffers

Buffer solutions are necessary to maintain the pH range of biological fluids.

Uses of Buffers

 

·     Buffer solutions are necessary to maintain the pH range of biological fluids. A buffer of carbonic acid (H2CO3) and bicarbonate (HCO3) is present in blood plasma, to maintain a pH between 7.35 and 7.45.

 

·     Enzymes are functional only under narrow pH range.

 

·     In Industries, buffer solutions are used in fermentation processes and in setting the correct conditions for dyes used in coloring fabrics.

 

·     They are also used for the calibration of pH meters.

 

·     Buffers are mainly used in clinical and research laboratories. Example., phosphate buffered saline (PBS) at pH 7.4.

 

Determination of pH : pH meter

 

A pH meter is used to measure the pH of a solution. It consists of a voltmeter attached to a pH-responsive electrode and a reference electrode. The difference in electrical potential produced by hydrogen ions between pH electrode and a reference electrode is measured. A pH meter is utilized in different laboratories and industries to measure pH.

 

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