Types of Mutations

Types of Mutations

The base sequence of DNA determines the amino acid sequence of a protein. The chemical and physical properties of each protein are determined by its amino acid sequence, so a single amino acid change is capable of altering the activity of, or even completely inactivating, a protein. Genotype refers to the genetic composition of an organism. Phenotype is an observable property of organism. The functional form of a gene is called Wildtype because presumably this is the form found in nature.

Mutation is the process by which the sequence of base pairs in a DNA molecule is altered.The alteration can be a single base pair substitution, insertion or deletion.

Mutations can be divided into two general categories:

1. Base–pair substitution mutation involves a change in the DNA such that one base pair is replaced by another.

• A mutation from one purine – pyrimidine base pair to the other purine –pyrimidine base pair is a transition mutation (Figure 12.7a). E.g. AT to GC, CG to TA.

• A mutation from a purine - pyrimdine base pair to a pyrimidine purine base pair is a transversion mutation (Figure 12.7b). E.g. AT to TA, CG to GC.

2. Base pair insertion or deletions  involves the addition or deletion of one base pair. If one or more base pairs are added to or deleted from a protein coding gene, the reading frame of an mRNA can change downstream of the mutation. An addition or deletion of one base pair, for example, shifts the mRNA’s downstream reading frame by one base, so that incorrect amino acids are added to the polypeptide chain after the mutation site. This type of mutation, called a frame shift mutation (Figure 12.8) usually results in a nonfunctional protein.

Frame shift mutations

• May generate new stop codons, resulting in a shortened protein.

• May result in a read through of the normal stop codon, resulting in longer than normal proteins

• Or may result in a complete alteration of the amino acid sequence of a protein.

Point mutations are single base changes, that do not affect the reading frame, that is, the mutation only makes a single change in a single codon, and everything else is undisturbed.

Mutations can also be defined according to their effects on amino acid sequences in proteins. They are

1. A missense mutation (Figure 12.9a) is a gene mutation in which a base – pair change in the DNA changes a codon in an mRNA so that a different amino acid is inserted into the polypeptide.

2. A neutral mutation (Figure 12.9b) is a subset of missense mutations in which the new codon codes for a different amino acid that is chemically equivalent to the original and therefore does not affect the proteins function. Consequently, the phenotype does not change.

 3. A silent mutation (Figure 12.9c) is also a subset of missense mutations that occurs when a base – pair change in a gene alters a codon in the mRNA such that the same amino acid is inserted in the protein. In this case, the protein obviously has a wild type function.

4. A nonsense mutation (Figure 12.9d) is a gene mutation in which a base – pair change in the DNA, changes a codon in an mRNA to a stop (nonsense) codon (UAG, UAA or UGA). Nonsense mutation cause premature chain termination so instead of complete polypeptides, shorter than normal polypeptide fragments (often nonfunctional) are formed.

Forward mutations change the genotype from wild type to mutant and reverse mutations (or reversions or back mutations) change the genotype from mutant to wild type or to partially wild type. An organism which has reverted is a Revertant. The effects of mutation may be diminished or abolished by a suppressor mutation. Suppressor mutation is a mutation at a different site from that of the original mutation. A suppressor mutation masks or compensates for the effects of the initial mutation, but it does not reverse the original mutation.