Microbial Genetics

Microbial Genetics

Learning Objectives

After studying this chapter the students will be able to,

• Define gene, genome, genetic code, genotype, phenotype, mutagen, wildtype.

• Describe transcription and translation.

• Classify mutations and its types and Understand how mutants are formed.

• Know the mode of action of physical and chemical mutagens.

• Identify the purpose of and outline the procedure for Ames test.

• Compare the gene transfer mechanisms.

• Know the types of cloning vectors used in genetic engineering.

• Describe how plasmids and bacteriophages are used to transfer foreign DNA.

• Explain the role of restriction enzymes in recombinant DNA technology.

• Know the types of restriction enzymes.

• Understand agarose gel electrophoresis and PCR techniques.

• Explain RAPD and RFLP.

Chapter Outline

1. Concept of Gene

2. Transcription

3. Genetic Code

4. Translation

5. Types of Mutation

6. Formation of Mutants

7. Transfer of Genetic Material

8. Recombinant DNA Technology

9. Vectors – Types and Characteristics

10. Restriction Enzymes

11. Techniques in Genetic Engineering