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The growing therapeutic use of proteins has created an increasing need for practical and economical processing techniques. As a result, biotechnological production methods have advanced tremendously in recent years, particularly in areas where disposable, single use production technology can be applied to mitigate many of the economic and quality issues arising from manufacturing these products (Hodge, 2004).
When producing proteins for therapeutic use, a number of issues must be considered related to the manufacturing, purification and characterization of the products. Biotechnological products for therapeutic use have to meet strict specifications, especially when used via the parenteral route (Walter and Werner, 1993).
In this chapter several aspects of production and purification will be dealt with briefly. For further details the reader is referred to the literature mentioned.
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