Suprapubic aspiration of urine
Optimal method for obtaining urine
for bacteriology in a child <2yr old.
•
21–23G
needle.
•
2–5mL
syringe.
•
An
alcohol impregnated swab.
•
Sterile
urine sample container.
•
Cotton
wool or gauze swab and adhesive plaster.
•
Wait
at least 30min from last urination.
•
If in
doubt as to whether the bladder contains adequate urine, perform bladder US to
confirm.
•
Place
child supine (with an assistant holding hips abducted) and then identify
site—midline anterior lower abdominal wall 1cm above the pubic bone.
•
Clean
site with an alcohol impregnated swab.
•
Insert
needle connected to the syringe at 90° to the skin, aspirating continuously until urine is obtained.
•
Insert
to almost the depth of the needle. If no urine is obtained, partially withdraw it before inserting again at different
angle.
•
Once
required urine is aspirated, remove the needle, press on puncture site with
cotton wool or gauze swab, and then apply an adhesive plaster.
•
Place
urine in sterile container.
•
If
unsuccessful, repeat the procedure 30–60min later.
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