BIOCHEMICAL
TECHNIQUES
Introduction
Biochemical
techniques involve the use of methods to evaluate the composition and
properties of biological samples. It has applications in different areas of
biological sciences. The information gained from such analysis provide
indication of the substances that are present (qualitative) or may specify the
exact amount of the substances (quantitative) present in the sample.
In
recent years, many methods have been developed for a widespread of analysis and
are marketed as assay kits. The kits used as diagnostic tools range from some
simple colorimetric assays to more sophisticated procedures involving
antibodies or nucleic acids. The kits include all necessary standards and can
be used in manual procedures or using an automated instrument. In biochemical
and research laboratories, a biochemist should be able to efficiently handle
techniques such as chromatography, centrifugation, electrophoresis and
spectrophotometry as they are the major techniques used in biochemistry.
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