Mikhail Tsvet, a Russian Botanist invented chromatography in the year 1906, which is derived from the Greek word meaning colour (chroma) and writing (graphein). It is an important technique of identifying, separating and purifying different properties of chemical components. Two main approaches of chromatography are analytical and preparative. Analytical methods uses small amount of sample to identify or characterise them. Preparative methods use larger quantities of samples and are mainly used for isolation process.