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Chapter: Microbiology and Immunology: Virology, Virus: Herpesviruses

Virus Isolation and Animal Susceptibility - Herpes Simplex Virus

The virus grows on the chorioallantoic membrane of the embryonated egg and produces small, white, shining, non-necrotic pocks measuring less than 0.5 mm in diameter.

Virus Isolation and Animal Susceptibility

 Chick embryo

The virus grows on the chorioallantoic membrane of the embryonated egg and produces small, white, shining, non-necrotic pocks measuring less than 0.5 mm in diameter. Both HSV-1 and HSV-2 show cross-reaction.

 Cell culture

Herpes simplex virus grows in a variety of primary and contin-uous cell lines. The viruses grow readily on HeLa cells, Hep-2 cells, human embryonic fibroblasts, and rabbit kidney cells.


      A cell line that expresses beta galactosidase after infection with HSV is a new approach for isolation and identification of the virus in the cell lines. Addition of a chromotropic substrate allows the detection of enzymes in the infected cell.


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Microbiology and Immunology: Virology, Virus: Herpesviruses : Virus Isolation and Animal Susceptibility - Herpes Simplex Virus |


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