Take one or two drops of butter milk on a slide and spread it. Heat the slide slightly on a lamp ( 3 – 4 seconds). Add a few drops of crystal violet and leave it for 30 to 60 seconds. Then wash the slide gently with water. Observe the slide under the compound microscope.
Take one or two drops of hay (In tamil, vaikol) decoction on a slide and observe it under the microscope.