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Geographical Distribution, Habitat, Morphology, Life Cycle, Pathogenicity, Clinical Manifestations, Laboratory Diagnosis, Treatment, Prevention and Control - Intestinal Flagellates - Giardia Lamblia | 12th Microbiology : Chapter 8 : Medical Parasitology

Chapter: 12th Microbiology : Chapter 8 : Medical Parasitology

Intestinal Flagellates - Giardia Lamblia

Intestinal Flagellates – Giardia Lamblia - Also known as Giardia duodenalis, Giardia intestinalis - 1. Geographical Distribution, 2. Habitat, 3. Morphology, 4. Life Cycle, 5. Pathogenicity, 6. Clinical Manifestations, 7. Laboratory Diagnosis, 8. Treatment, 9. Prevention and Control

Intestinal Flagellates – Giardia Lamblia

(Also known as Giardia duodenalis, Giardia intestinalis)

 

Geographical Distribution

It is the most common protozoan pathogen and is worldwide in distribution. The disease is very high in areas with low sanitation, especially tropics and subtropics.

 

Habitat

Giardia lamblia lives in the duodenum and upper jejunum of human. It is the only protozoan parasite found in the lumen of the human small intestine.

 

Morphology

It exists in two forms

• Trophozoite and

• Cyst

Antoine van Leeuwenhoek observed and illustrated Giardia lamblia in his own loose stool. This was the first protozoan parasite of human that is recorded and the first to be seen under a microscope.

Trophozoite

The trophozoite is in the shape of a tennis or badminton racket. It is rounded anteriorly and pointed posteriorly. The size of the trophozoite is 14 µ long by 7µ broad. Dorsally, it is convex and ventrally, it has a concave sucking disc which helps in its attachment to the intestinal mucosa. It is bilaterally symmetrical. All the organs of the body are paired. Trophozoite of Giardia possess,

• 1 pair of nuclei

• 4 pairs of flagella

•  Parabasal body (Blepharoplast), from which the flagella arise (4 pairs)

•  1 pair of axostyles, running along the midline

• Two sausage – shaped parabasal or median bodies lying transversely posterior to the sucking disc

• The trophozoite is motile, with a slow oscillation about its long axis, often resembling falling leaf (Figure 8.6a).

Cyst

It is the infective form of the parasite. The cyst is small and oval, measuring 12 µm × 8 µm and is surrounded by a hyaline cyst wall.

Its internal structure includes 2 pairs of nuclei grouped at one end. A young cyst contains 1 pair of nuclei. The axostyle lies diagnonally, forming a dividing line within cyst wall (Figure 8.6b).


 

Life Cycle:

Giardia Life Cycle in Host (Human)

Infective form: Mature cyst

Mode of transmission: Human acquires infection by ingestion of cyst in contaminated water and food. Direct person – to person transmission occurs in children. Transmission occurs through oral-anal and oral-genital route in sexually active homosexual males. Within half an hour of ingestion, the cyst hatches out into two trophozoites, which multiply by binary fission and colonize in the duodenum. The trophozoites live in the duodenum and upper part of jejunum, feeding by pinocytosis. When conditions in duodenum are unfavourable, encystment occurs, usually in large intestine. Cysts are passed in stool and remain viable in soil and water for several weeks (Figure 8.7)


 

Pathogenicity

Giardia lamblia does not invade the tissue, but remains attached to intestinal epithelium by means of the sucking disc. It causes a disturbance of intestinal function leading to malabsorption of fat

 

Clinical Manifestations

Incubation period is variable, but is usually about 2 weeks.

The disease is asymptomatic, but in some cases it may lead to abdominal cramps, flatulence, looseness of bowels, foul smelling stool and mild steatorrhoea (passage of yellowish and greasy stools in which there is excess of fat). The stool contains excess mucus and fat but no blood and pus. Children may develop chronic diarrhoea, malaise (discomfort), nausea, anorexia (loss of appetite for food), malabsorption of fat, vitamin A and protein. Occasionally, Giardia may colonize the gall bladder causing biliary colic and jaundice.

 

Laboratory Diagnosis

Specimens: Stool and blood

Examination of stool sample: Giardiasis can be diagnosed by identification of cysts of Giardia lamblia in the formed stools and the trophozoites and cyst of the parasite in diarrhoeal stools.

Macroscopic examination of stool: Fecal specimens containing Giardia lamblia may have an offensive odor. It is pale coloured with fatty substance floating in water.

Microscopic examination of stool: Cysts and trophozoites can be found in diarrheal stools by saline and iodine wet preparations (Figure 8.8).


Serodiagnosis: Immuno chromatographic strip tests and indirect immunofluorescence (IIF) tests are readily available. For antigen and antigen detection ELISA, Commercially available ELISA kits detects Giardia – Specific antigen.

Molecular methods: DNA probes and polymerase chain reaction (PCR) have been used to demonstrate parasitic genome in the stool specimen

 

Treatment

Metronidazole and Tinidazole are the drugs of choice.

 

Prevention and Control

Giardiasis can be prevented and controlled by,

• Proper disposal of human faeces, maintenance of food and personal hygiene and health education.

• After using the bathroom and before eating, the hands should be washed thoroughly with soap and warm water. Boiling of water is the best and effective method in killing the viable cysts.

• To reduce the risk of venereal transmission, patients should avoid risky sexual behavior.

• No vaccine or effective chemo prophylactic drug is available for prevention of Giardiasis..

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