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Chapter: 11th Microbiology : Stains and Staining Methods

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Classification of Stains

1. On the basis of origin, stains can be classified as natural and synthetic. 2. On the basis of chemical behavior, dyes are classified as acidic, basic and neutral.

Classification of Stains

 

1. On the basis of origin, stains can be classified as natural and synthetic.

i. Natural stains:

·        These stains are obtained directly from natural products. For example, Haematoxylin is obtained from the heartwood of a tree (Haematoxylon campechianum).

·        The natural stains are used mainly for histological purposes.

ii. Synthetic stains:

·        These are artificially produced mainly from coal tar products and hence popularly called coal-tar dyes.

·        A majority of stains used in microbiology are the synthetic type and manufactured from Aniline. For example, Crystal violet, Safranin, Methylene blue and Acid fuchsin.

 

2. On the basis of chemical behavior, dyes are classified as acidic, basic and neutral.

·        An acidic dye is one in which the colour bearing ion, the chromophore, is an anion.

·        A basic dye is one in which the colour bearing ion, the chromophore, is a cation.

·        A neutral dye is a complex salt of a dye acid with a dye base.

Acid dyes generally combine more strongly with cytoplasmic (basic) elements of the cell, and basic dyes combine best with nucleic acid (acidic) elements of the cell. Table 3.2 shows the chemical characteristics of a stain or dye.

 

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