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Chapter: 11th Microbiology : Agricultural Microbiology

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Biogeochemical Cycles

Biogeochemical cycling is defined as the movement and conversions of materials by biochemical activities throughout air, water and soil.

Biogeochemical Cycles

 

Biogeochemical cycling is defined as the movement and conversions of materials by biochemical activities throughout air, water and soil. All living organisms participate in biogeochemical cycling but microorganisms play a major role. This is because of their high enzymatic activity and their ubiquity.

 

Most macro elemental components of living organisms like Carbon, Nitrogen, Sulphur, Oxygen, Phosphorus and Hydrogen are cycled most intensely and other elements like Copper, Chromium, Iron, Zinc, Nickel are cycled less intensely.

 

Carbon Cycle

Carbon is a macro element present in all living cells. In microorganisms, they are present in all macromolecules like cell wall, cytoplasmic membrane, proteins and nucleic acids.

 

Reservoirs of Carbon:

Reservoirs are the storage places of nutrients that are present in nature. They store nutrients in large amounts for longer periods of time.

Atmospheric CO2, dissolved carbon in oceans and freshwater, organic matter are actively cycled carbon reservoirs. Sediments and fossil fuels like coal, petroleum and natural gas are slowly cycled carbon reservoirs. Carbon is cycled between these reservoirs by the biochemical activities of micro organisms and other living things (Figure 11.1).


 

The different stages or processes involved in carbon cycle are

1. Photosynthesis

2. Decomposition

3. Methanogenesis

 

1. Photosynthesis

It is a process where atmospheric CO2 is converted to organic carbon (CH2O)n. This is carried out by higher plants, photosynthetic bacteria, cyanobacteria and algae using radiant energy from the sun. This can be explained by the equation,


where (CH2O)n represents the organic form of carbon (Example: Carbohydrates) which gets incorporated into the photosynthetic organisms. This organic carbon serves as food for herbivores and in turn for carnivores.

 

2. Decomposition

The organic matter fixed as a result of photosynthesis is eventually degraded by microorganisms to CO2 during processes like respiration and decomposition. When aerobic and anaerobic organisms respire, CO2 is released into the atmosphere. Much of the CO2 is released when dead organisms decompose in the soil predominantly by the activities of soil microorganisms. Burning of fossil fuels also release CO2 into the atmosphere.

 

3. Methanogenesis

It is an anaerobic process where CO2 gets converted to CH4 (methane) by strict anaerobes like methanogens (Example: Methanobacterium). Methanogens are a group of Archaebacteria found in anaerobic environments like swamps, marshes, rumen of ruminants, paddy fields and gut of termites.


Methane is converted back to carbondioxide by a process called Methylotrophy.

 

Nitrogen Cycle


The element Nitrogen (N) is a key constituent in microbial cell. Nitrogen is needed for the formation of proteins, amino acids, nucleotides and is present in a number of oxidation states inside the cell. Nitrogen is cycled between atmosphere, organic compounds in living things, soil and sediments.

 

The processes that are involved in Nitrogen cycle are

1.     Nitrogen fixation

2.     Nitrification

3.     Ammonification

4.     Denitrification

 

Nitrogen fixation

Nitrogen is present as N2 (N  N) in air (78% N2). The triple bonded state of nitrogen makes it very stable and nitrogen in its gaseous state cannot be assimilated by plants or animals for their metabolism. Only few groups of prokaryotes are capable of breaking the triple bond and use it for building up their proteins and aminoacids. The process of reduction of gaseous nitrogen (N2) to ammonia (NH4) is called Nitrogen fixation. This process is carried out by a group of prokaryotes called diazotrophs.


Cyanobacteria, Rhizobium and Frankia are some of the examples of diazotrophs that can fix atmospheric nitrogen. The fixed ammonia gets incorporated into proteins and amino acids, thus building up organic nitrogen.


 

Ammonification

The production of ammonia during the decomposition of organic nitrogen compounds, by micro organisms after the death of plants and animals is called ammonification (Figure 11.2). Much of the ammonia released by aerobic decomposition in soil is taken up rapidly by plants and micro organisms and is converted to amino acids.


Bacteria like Bacillus, Clostridium, Pseudomonas and fungi like Aspergillus, Mucor and Penicillium are few examples of micro organisms that can ammonify.

 

Nitrification

The oxidation of ammonia (NH3) to nitrate (NO3) is called nitrification. It is carried out by nitrifying bacteria. It is a two step process where ammonia is first converted to nitrite (NO2) and then to nitrate (NO3).

2NH3 + 3O2 → 2HNO2 + 2H2O

The above given oxidation reaction is the first step that produces nitrite. This reaction provides energy and is carried out by Nitrosomonas and Nitrosococcus.

In the second step, the nitrite is oxidized to nitrate

2HNO2 + O2 → HNO3

This reaction is carried out by Nitrobacter.

Nitrates are readily assimilated by plants but are very water soluble and rapidly leached from soil.

 

Denitrification

The reduction of NO3- from soils by denitrifying bacteria to gaseous nitrogen is called denitrification. In this process, carried out by bacteria like Pseudomonas,

Thiobacillus denitrificans, organic compounds serve as hydrogen donors and nitrates serve as electron acceptor.

NO3 → NO2 → N2O → N2

 

Biological Nitrogen Fixation

One of the most significant biological process taking places on the Earth is biological nitrogen fixation (BNF). This fixation of atmospheric nitrogen carried out by few prokaryotes is cost efficient because industrial production of ammonia by Haber’s Bosch process is very expensive.

Organisms carry out BNF in a free living state in soil or they can establish symbiotic association with other plants or micro organisms and fix N2.

Organisms capable of BNF are


 

BNF by Rhizobium in leguminous plants

Leguminous plants belong to the family Leguminaceae and bear seeds in pods. Example: Black gram, Green peas, Soyabean, Subabul. The bacteria belonging to the genus Rhizobium which can exist in free living state in soil but can enter into symbiosis with legume plants and carry out nitrogen fixation.

 

Process of BNF

It consists of the following steps

1. Infection of legume roots by Rhizobium

2. Formation of root nodules

3. Reduction of N  N to NH4 in root nodules

 

1. Infection of legume roots by Rhizobium

Rhizobium recognises and attaches to the root hairs of legume plant. It invades the root hairs and secretion of certain nod factors result in root hair curling typically called Shepherd’s crook symptom which leads to the formation of infection thread. Infection thread is a cellulosic tube like structure through which Rhizobium moves into the cortex from root hairs.

 

2. Formation of root nodules

The invaded plant cells are stimulated to divide repeatedly thus forming a mass of tissue on the roots which are called root nodules (Figure 11.3). Root nodules are fleshy light pink colored globose structures seen on the roots. The bacteria inside the root nodules transform into swollen, mishappened forms. These are called bacteroids. The bacteroids are capable of nitrogen fixation (Figure 11.4)




 

3. Reduction of NN to ammonia in root nodules

This biochemical process is catalysed by an enzyme called Nitrogenase present in bacteroids and happens under diminished O2 levels. The O2 levels in the nodules are controlled by an oxygen binding protein called leghemoglobin. This is a red, iron containing protein which can keep the nodule environment free of oxygen.

 

Nitrogenase

The enzyme nitrogenase is a complex enzyme consisting of 2 enzymes, dinitrogenase reductase and dinitrogenase. Electrons from organic compounds like pyruvate are passed on to dinitrogenase reductase first and then to dinitrogenase which in turn passes them to N  N thus reducing it to NH4. This reduction needs 16 ATP, ferredoxin and cytochromes.


 

Phosphorus Cycle


The element phosphorus (P) is an essential macro element in all living organisms. They are found in nucleic acids and phosphate esters. It is an essential component of ATP and other high energy phosphates and phospholipids.

 

Reservoirs of Phosphorus

1. Phosphate rock like apatite, a large inert reservoir

2. Marine and aquatic sediments

3. Dissolved phosphates in soils and waters

4. Organic phosphates in dead and living organisms.

Phosphorous transformations mostly happen as inter conversion of inorganic to organic phosphate and insoluble form to soluble phosphates.


 

Phosphate solubilizations

Most of phosphates occur in combination with Calcium, Iron, Magnesium and Aluminium (inorganic P) and thus are insoluble and unavailable to plants and micro organisms. Some micro organisms solubilize those insoluble phosphates by producing organic acids. Example: Thiobacillus, Bacillus thus enabling the plants to utilize it.

 

Phosphate assimilation

Plants and micro organisms can readily assimilate soluble forms of inorganic phosphates like H2PO4-, HPO4-2 and HPO4-3 and incorporate them as organic forms of phosphates like ATP, nucleic acids.

 

Phosphate mineralisation

Breakdown of organic phosphates to form soluble inorganic phosphates is called mineralisation. Organisms produce phosphatase enzymes and catalyse mineralisation. The mineralised phosphates can be utilized by plants (Figure 11.5).


 

Sulphur Cycle


Sulphur is present in sulphur containing aminoacids. The sulphur cycle involves oxidation – reduction reaction between Sulphate (SO4), Elemental S and H2S and hence there is change in the valence states of sulphur from -2 to +6.

The basic steps involved in sulphur cycle are

1.     Sulphide/ sulphur oxidation

2.     Sulphate reduction

3.     Sulphur reduction

4.     Organicsulphur compound oxidation or reduction

5.     Desulfurylation

 

Sulphide/Sulphur oxidation (H2S→S0→SO42-)

It is carried out by prokaryotes under aerobic and anaerobic conditions. Under aerobic conditions, H2S is spontaneously oxidized at neutral pH to elemental sulphur. Elemental sulphur is oxidized to suphates by chemolithotrophic bacteria like Thiobacillus, Beggiatoa.

If light is available, H2S can be used as electron donor to carry out photosynthesis under anoxic conditions by phototrophic sulphur bacteria like Chromatium and Chlorobium.

 

Sulphate reduction

When sulphate is present in habitats, different groups of microorganisms are capable of carrying out sulphate reduction.

Beijerinck described the use of sulphate (SO42-) as a terminal electron acceptor during anaerobic respiration to form sulphide (H2S). This process is called Dissimilatory Sulphate Reduction(DSR). The anerobic bacteria capable of carrying out DSR are Desulfovibrio, Desulfococcus, Desulfotomaculum (Figure 11.6) This reaction by sulphate reducers requires organic carbon sources like pyruvate or lactate. H2S accumulated in such habitats by the action of sulphate reducers is toxic to aerobic organisms.

The reduction of sulphate to H2S, for building up aminoacids and proteins


is called as assimilatory sulphate reduction. The H2S thus produced is immediately incorporated into organic compounds.

 

Sulphur reduction (S0→H2S):

 

The dissimilative sulphur reducing bacteria can reduce elemental sulphur to hydrogen sulphide. Example: Desulfuromonas, an obligate anaerobe. Under aerobic conditions, organisms like Pseudomonas, Proteus and Salmonella are also capable of performing this reaction.

 

Organic sulphur compounds reduction/oxidation

Organic sulphur compounds like dimethyl sulphide can be used as carbon and energy source for many microorganisms.

 

Desulfurylation

It is a process where organic sulphur compounds are used up by microorganisms for energy to produce H2S. 

 

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