The term
absorption refers to complete retention of light, without reflection or
transmission. Pigments absorb different wavelengths of light. A curve obtained
by plotting the amount of absorption of different wavelengths of light by a
pigment is called its absorption
spectrum.
• Chlorophyll 'a' and chlorophyll 'b' absorb quanta from blue and red region
• Maximum absorption peak for different forms of chlorophyll 'a' is 670 to 673, 680 to 683 and 695 to 705nm.
• Chlorophyll 'a' 680 (P680) and Chlorophyll 'a' 700 (P700) function as trap centre for PS II and PS I respectively.
The
effectiveness of different wavelength of light on photosynthesis is measured by
plotting against quantum yield. The curve showing the rate of photosynthesis at
different wavelengths of light is called action
spectrum. From the graph showing action spectrum, it can be concluded that
maximum photosynthesis takes place in blue and red region of the spectrum. This
wavelength of the spectrum is the
absorption maxima for Chlorophyll (a) and Chlorophyll (b). The Action Spectrum
is instrumental in the discovery of the existence of two photosystems in O2
evolving photosynthesis (Figure 13. 8).
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