Replication
It is a process in which DNA copies itself to
produce identical daughter molecules of DNA.
Models
of Replication
Three models of replication had been proposed.
They are 1. Conservative replication 2.Dispersive replication and 3.Semi
conservative replication.
According to this model, the parental DNA is
conserved to one daughter cell and the newly synthesized DNA to another
daughter cell.
According to this model, the parental DNA is
unequally distributed (randomly) to the daughter cells.
Semiconseravative model of replication showing
the daughter DNA having one parental strand and one daughter strand.
This model was established to be the correct
mode of replication by the experiment carried out by Messlson and Stahl in
1957. E. coli cells were grown in a
medium containing 15NH4Cl, for many generations such that the E.coli
cells have density labelled 15N atoms in their DNA. The cells were then grown
in a medium containing unlabelled NH4Cl. DNA was harvested from the
daughter cells after one generation and the density of the DNA was analyzed
using Cesium chloride density gradient centrifugation. If replication happens
to occur by conservative model, two bands are corresponding to heavy DNA and
other unlabelled DNA should be got. But if replication is semi-conservative,
daughter DNAs should possess one labelled strand and one unlabelled strand, so
they will form only one band with intermediate density. The results show that
they form bands with intermediate density alone in the first generation, which
confirms the semiconservative model of replication.
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