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Chapter: Microbiology and Immunology: Virology, Virus: Orthomyxoviruses

Virus Isolation and Animal Susceptibility - Influenza Viruses

Influenza viruses are isolated from respiratory secretions by growing in chick embryos or tissue cultures (monkey kidney or baboon kidney cell lines).

Virus Isolation and Animal Susceptibility

Influenza viruses are isolated from respiratory secretions by growing in chick embryos or tissue cultures (monkey kidney or baboon kidney cell lines).

 Chick embryos

The influenza viruses grow in the allantoic and amniotic cavity of the chick embryos. After an incubation period of 3 days, the fluid is tested for hemagglutination activities of the viruses.

 Cell culture

Cell lines are widely used for culture of influenza viruses. They can grow in several primary and continuous cell lines. Rhesus Monkey kidney cell lines (LLC-MK2) and Madin–Darby canine kidney (MDCK) are the continuous cell lines frequently used to isolate influenza viruses.

 Laboratory animals

Human influenza virus causes experimental infections in a variety of animals. Intracerebral inoculation of mice by neuro-trophic strains produces fatal encephalitis. It causes an acute respiratory disease on intranasal inoculation in ferrets.

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Microbiology and Immunology: Virology, Virus: Orthomyxoviruses : Virus Isolation and Animal Susceptibility - Influenza Viruses |


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