Mixing and Shaking
Improper handling of protein products can lead to denaturation. Shaking and severe agitation of most of these products will result in degradation. Therefore special techniques must be observed in preparing biotech products for use. Biotech products should not be shaken when adding any diluent as this may cause the product to break down. Once the diluent is added to the container, the vial should be swirled rather than vigorously shaken. Some shaking during transport may be unavoidable and proper inspection of products should occur to make sure the products have not been damaged during transit. When a product is affected by excessive shaking, physical separation or frothing within the vial of liquid products can usually be observed. For lyophilized products, agitation is not harmful until the product has been reconstituted. In distributing individual products to patient or ward areas, pneumatic tubes should be avoided.
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