Experiment to show the germination of pollen grains to form pollen tube

Experiment to show the germination of pollen grains to form pollen tube:

Requirements: Sucrose (a sugar) solution, Sodium borate solution, slide,Microscope, fresh pollen grains; and a petridish.

Procedure: Let us take a little amount (10-15 parts) of sucrose solution in apetridish. 0.01 % sodium borate solution is then mixed with sucrose solution.

On this mixture a good amount of fresh pollen grain is then spread over. In this condition the set is left for one day.

Observation: After one day let the petridish beobserved under simple microscope. It will be seen  a  good  number  of  pollen  grain  developspollen tubes on germination.If you can collect pollen grain of Gima Shak by10 o'clock in the morning, you will be able toobserve germinating pollen tubes. Most pollen grain germinates in 10 -20% sugar solution. So if you do not have sodium borate, you can do this experiment with sugar solution. In most cases germination may be observed if pollen grains are collected after a few hours of dehiscence of anther.