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Chapter: Microbiology and Immunology: Virology, Virus: Paramyxoviruses

Laboratory Diagnosis - Respiratory Syncytial Infections

These include respiratory secretions obtained by washing, suc-tioning, or swabbing of the nasopharynx.

Laboratory Diagnosis

 Specimens

These include respiratory secretions obtained by washing, suc-tioning, or swabbing of the nasopharynx.

 Direct antigen detection

The viral antigens in the nasal washings or nasopharyngeal aspirates can be detected by using ELISA or direct immunoflu-orescence antibody test using specific monoclonal antibodies.

 Isolation of the virus

The virus can be isolated from clinical specimens by inocula-tion of HeLa cells, Hep-2 cells, or monkey kidney cells. After 2–10 days of incubation, RSV is identified by the character-istic syncytium formation in the cells. Direct immunofluo-rescence can also be used to detect viral antigen in infected cells.

 

 Serodiagnosis

The ELISA is used for demonstration of antibodies in the serum. Demonstration of a fourfold or more increase in the antibody titer of acute and convalescent sera confirms the diagnosis of RSV infection.

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Microbiology and Immunology: Virology, Virus: Paramyxoviruses : Laboratory Diagnosis - Respiratory Syncytial Infections |


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