Equipments
Confocal Microscopy
Confocal
microscopy offers several advantages over conventional optical microscopy,
including shallow depth of field, elimination of out-of-focus glare, and the
ability to collect serial optical sections from thick specimens. In the
biomedical sciences, a major application of confocal microscopy involves
imaging either fixed or living cells and tissues that have usually been labeled
with one or more fluorescent probes.
Microbiome
The human microbe ome is composed of communities of bacte-ria,
viruses and fungi that have a greater complexity than the human genome it-self.
Genome sequencing technologies and metagenomic analysis has helped in our
understanding of human micro-biome. This is useful in manipulation of gut
microbiome to be used in the treat-ment of childhood diseases.
Confocal
microscopy, most frequently confocal
laser scanning microscopy (CLSM) or laser confocal scanning microscopy (LCSM),
is an optical imaging technique for
increasing optical resolution and contrast of a micrograph by means of using a
spatial pinhole to block out-of-focus light in image formation. Capturing
multiple two-dimensional images at different depths in a sample enables the
reconstruction of three-dimensional structures (a process known as optical
sectioning) within an object. This technique is used extensively in the
scientific and industrial communities and typical applications are in life
sciences, semiconductor inspection and materials science
Light
travels through the sample under a conventional microscope as far into the
specimen as it can penetrate, while a confocal microscope only focuses a
smaller beam of light at one narrow depth level at a time. The CLSM achieves a
controlled and highly limited depth of focus.
DNA Sequencing System
Sequencing
means finding the order of nucleotides on a piece of DNA. Nucleotide order
determines amino acid order, and by extension, protein structure and function
(proteomics). An alteration in a DNA sequence can lead to an altered or non
functional protein, and hence to a genetic disorder. DNA sequence is important
to detect the type of mutations in genetic diseases and offer hope for the
eventual development of treatment DNA.
Methods
of sequencing
1. Sanger
dideoxy (primer extension/ chain-termination) method
Most
popular protocol for sequencing, very adaptable, scalable to large sequencing
projects.
2. Maxam-Gilbert chemical cleavage method
DNA is
labelled and then chemically cleaved in a sequence dependent manner. This
method is not easily scaled and is rather tedious.
It
provides an important tool for determining the thousands of nucleotide
variations associated with specific genetic diseases, like Huntington’s, which
may help to better understand these diseases and advance treatment.
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