Difference
between light and electron microscope
1. Light
is the illuminating source
2.
Specimen preparation takes usually few minutes to hours. Live or dead specimen
may be seen
3.
Condenser, objective and eye piece lenses are made up of glasses
4.
Specimen is stained by coloured dyes
5. It has
low resolving power (0.25µm to 0.3 µm). It has a magnification of 500X to 1500X
6. Vacuum
is not required
7. Image
is seen by eyes through ocular lens
1. The
beam of electrons is the electron source
2.
Specimen preparation takes usually takes a few days. Only dead or dried
specimen are seen
3. All
lenses are electromagnetic
4.
Specimen is coated with heavy metals in order to reflect electrons
5. It has
high resolving power (0.001µm), about 250 times higher than light microscope.
It has a magnification more than 100,000X
6. Vacuum
is essential for its operation
7. Image
is produced on fluorescent screen or photographic plate
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